PXD027855

PXD027855 is an original dataset announced via ProteomeXchange.

Title	Comparison of Cell Wall Proteomics of Drug Resistant M. smegmatis with and without Sub-inhibitory Rifampicin Exposure
Description	We previously showed sub-lethal exposure to the front-line anti-TB drug rifampicin resulted in substantial adaptive remodelling of the proteome of the model organism M. smegmatis in the drug sensitive mc2155 strain (WT). Here we investigate whether these responses are conserved in an engineered, isogenic M. smegmatis mc2155 mutant harbouring the clinically relevant S531L rifampicin resistance-conferring mutation (SL) and distinguish responses that are specific to rifampicin’s anti-bacterial activity from those that are dependent on rifampicin's presence alone. We used a cell wall enrichment strategy to focus attention on the cell wall proteome and observed 253 proteins to be dysregulated in SL bacteria as compared with 716 proteins in WT. We observed that down regulation of ABC transporters and up regulation of ribosomal machinery were conserved in the SL strain, while up regulation of transcriptional machinery, and down regulation of numerous two-component systems, were not.
HostingRepository	PRIDE
AnnounceDate	2021-11-02
AnnouncementXML	Submission_2021-11-02_03:38:41.310.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexander Giddey
SpeciesList	scientific name: Mycolicibacterium smegmatis MC2 155; NCBI TaxID: 246196;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-11 04:27:15	ID requested
⏵ 1	2021-11-02 03:38:41	announced

Dataset with its publication pending

submitter keyword: Mass spectrometry, Proteomics, Rifampicin, Mycobacterium smegmatis, Phenotypic adaption, ABC transporters

Jonathan M Blackburn
contact affiliation	Department of Chemical and Systems Biology, Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, South Africa
contact email	jonathan.blackburn@uct.ac.za
lab head
Alexander Giddey
contact affiliation	University of Cape Town
contact email	gddale001@myuct.ac.za
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027855
     1. Label: PRIDE project
     2. Name: Comparison of Cell Wall Proteomics of Drug Resistant M. smegmatis with and without Sub-inhibitory Rifampicin Exposure