PXD027854

PXD027854 is an original dataset announced via ProteomeXchange.

Title	Enzymatic activity of glycosyltransferase GLT8D1 promotes human glioblastoma cells migration
Description	Glioblastoma (GBM) is the most aggressive primary brain tumor characterized by infiltrative migration of malignant glioma cells into the surrounding brain parenchyma. In this study, our analysis of GBM patient cohorts displayed significant higher expression of Glycosyltransferase 8 domain containing 1 (GLT8D1) compared to normal brain tissue and could be associated with impaired patient survival. Increased in vitro expression of GLT8D1 significantly enhanced migration of two different sphere-forming GBM cell lines. By in silico analysis we predicted the 3D-structure as well as the active site residues of GLT8D1. The introduction of point mutations in the predicted active site reduced its glycosyltransferase activity in vitro and consequently impaired GBM tumor cell migration. Examination of GLT8D1 interaction partners by LC-MS/MS implied proteins associated with cytoskeleton and intracellular transport as potential substrates. In conclusion, we demonstrated that the enzymatic activity of glycosyltransferase GLT8D1 promotes GBM cell migration.
HostingRepository	PRIDE
AnnounceDate	2022-05-27
AnnouncementXML	Submission_2022-05-27_02:51:42.093.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gunnar Dittmar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-11 04:27:14	ID requested
⏵ 1	2022-05-27 02:51:43	announced

Ilina EI, Cialini C, Gerloff DL, Duarte Garcia-Escudero M, Jeanty C, Th, é, z, é, nas ML, Lesur A, Puard V, Bernardin F, Moter A, Schuster A, Dieterle M, Golebiewska A, G, é, rardy JJ, Dittmar G, Niclou SP, M, ü, ller T, Mittelbronn M, Enzymatic activity of glycosyltransferase GLT8D1 promotes human glioblastoma cell migration. iScience, 25(2):103842(2022) [pubmed]

submitter keyword: GLT8D1, glioblastoma, human, Q-Exactive HF, interactome

Gunnar Dittmar
contact affiliation	Luxembourg instititute of health
contact email	gunnar.dittmar@lih.lu
lab head
Gunnar Dittmar
contact affiliation	LIH
contact email	gunnar.dittmar@lih.lu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD027854

PRIDE project URI

• PRIDE
  1. PXD027854
     1. Label: PRIDE project
     2. Name: Enzymatic activity of glycosyltransferase GLT8D1 promotes human glioblastoma cells migration