PXD027837

PXD027837 is an original dataset announced via ProteomeXchange.

Title	Formation and persistence of polyglutamine aggregates in mistranslating cells
Description	In neurodegenerative diseases, including pathologies with well-known causative alleles, genetic factors that modify severity or age of onset are not entirely understood. We recently documented the unexpected prevalence of transfer RNA (tRNA) mutants in the human population, including variants that cause amino acid mis-incorporation. We hypothesized that a mistranslating tRNA will exacerbate toxicity and modify the molecular pathology of disease-causing alleles and investigated the combinatorial effects of tRNA variants in cellular models of Huntington’s disease. This dataset represents MS/MS data confirming Phenylaline to Serine amino acid misincorporation events caused by the tRNA-Ser G35A variant described in our study. The wildtype (WT) or mistranslating tRNA (VAR) were expressed in murine N2a cells along with mCherry protein. We affinity purified the mCherry protein to search for Ser misincorporation events at Phe codons.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:32:52.598.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027837
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jeremy Lant
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	L-methionine sulfone; iodinated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-09 23:58:28	ID requested
1	2022-02-17 06:01:07	announced
⏵ 2	2024-10-22 05:32:58	announced	2024-10-22: Updated project metadata.

Lant JT, Kiri R, Duennwald ML, O'Donoghue P, Formation and persistence of polyglutamine aggregates in mistranslating cells. Nucleic Acids Res, 49(20):11883-11899(2021) [pubmed]

10.1093/nar/gkab898;

10.6019/PXD027837;

submitter keyword: tRNA, amino acid misincorporation, mistranslation

Patrick O'Donoghue
contact affiliation	Dept of Biochemistry, Medical Sciences Building, Schulich School of Medicince and Dentistry, University of Western Ontario, London, ON, Canada
contact email	podonog@uwo.ca
lab head
Jeremy Lant
contact affiliation	University of Western Ontario
contact email	jlant@uwo.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027837

PRIDE project URI

• PRIDE
  1. PXD027837
     1. Label: PRIDE project
     2. Name: Formation and persistence of polyglutamine aggregates in mistranslating cells