PXD027822

PXD027822 is an original dataset announced via ProteomeXchange.

Title	Label-free LC-MS/MS of Ubiquitinated proteins derived from UPs-4T1/WT and UPs-4T1/EPB nanovaccine
Description	In this project, 4T1 parental cells (4T1/WT) were exposed to increasing concentrations of epirubicin (EPB) to establish a novel multi-drug resistant CSC-like breast cancer cell line (4T1/EPB). The ubiquitinated proteins were enriched from 4T1/WT or 4T1/EPB derived cell lysate using a-Al2O3-Vx3 nanoparticles to produce the covalently linked product UPs nanovaccine. Label-free LC-MS/MS mass spectrometry was used to detect the type and amount of enriched proteins of UPs from the 4T1/WT cells and the 4T1/EPB cells.
HostingRepository	PRIDE
AnnounceDate	2021-08-20
AnnouncementXML	Submission_2021-08-20_09:30:38.529.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fang Huang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-09 06:35:02	ID requested
⏵ 1	2021-08-20 09:30:38	announced

Dataset with its publication pending

submitter keyword: mouse, Label-free LC-MS/MS, Ubiquitinated proteins, UPs-4T1/WT nanovaccine, UPs-4T1/EPB nanovaccine

Fang Huang
contact affiliation	Department of Microbiology and Immunology, Medical School of Southeast University, 87 Dingjiaqiao Rd, Nanjing, Jiangsu Province 210009, P. R. China.
contact email	596824235@qq.com
lab head
Fang Huang
contact affiliation	Department of Microbiology and Immunology, Medical School of Southeast University, 87 Dingjiaqiao Rd, Nanjing, Jiangsu Province 210009, P. R. China.
contact email	596824235@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD027822

PRIDE project URI

• PRIDE
  1. PXD027822
     1. Label: PRIDE project
     2. Name: Label-free LC-MS/MS of Ubiquitinated proteins derived from UPs-4T1/WT and UPs-4T1/EPB nanovaccine