PXD027819

PXD027819 is an original dataset announced via ProteomeXchange.

Title	Nanoparticles and photochemistry for Native-like transmembrane protein footprinting
Description	Mass spectrometry based footprinting can probe higher order structure of proteins. We bond photocatalytic nanoparticles to a lipid bilayer that, upon laser irradiation, produce high local concentrations of radicals that penetrate the lipid layer made permeable by a simultaneous laser-initiated PB reaction. This approach achieves high footprinting coverage for membrane proteins in liposomes, helps locate both ligand-binding residues in a transporter and ligand-induced conformational changes, and reveals structural aspects of the flexible unbound state. Overall, this approach proves highly effective in intramembrane footprinting and forges a connection between materials science and biology.
HostingRepository	PRIDE
AnnounceDate	2023-04-11
AnnouncementXML	Submission_2023-04-11_06:07:40.677.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JieSun
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-09 06:34:48	ID requested
1	2021-10-05 02:02:10	announced
⏵ 2	2023-04-11 06:07:41	announced	2023-04-11: Updated project metadata.

Sun J, Liu XR, Li S, He P, Li W, Gross ML, Nanoparticles and photochemistry for native-like transmembrane protein footprinting. Nat Commun, 12(1):7270(2021) [pubmed]

submitter keyword: footprinting,membrane protein

Michael L.Gross
contact affiliation	Department of Chemistry, Washington University in St. Louis
contact email	mgross@wustl.edu
lab head
JieSun
contact affiliation	Washington University in St. Louis
contact email	jie.sun@wustl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD027819

PRIDE project URI

• PRIDE
  1. PXD027819
     1. Label: PRIDE project
     2. Name: Nanoparticles and photochemistry for Native-like transmembrane protein footprinting