PXD027813

PXD027813 is an original dataset announced via ProteomeXchange.

Title	Comparative LC-MS analysis of two metronidazole resistant (M1,M2) and one revertant (M1NR) Giardia intestinalis strains with their parental strain (WB-C6)
Description	I this study we analyzed three lines, two of which were created by UV- directed mutagenesis and showed increased levels of metronidazole tolerance (M1 and M2) and the third being a line that reverted spontanously to sensitive after several passages (M1NR) of M1 without metronidazole in the media. On the physiological side we analyzed the ability of these lines to reists metronidazole exposure and to encyst as well as their respective doublings times. At the same time we conducted a trasncriptomics and proteomics analysis to understand which exact changes lead to M1 and M2 being resistant while M1NR shows wildtype levels of metrondiazole sensitivty. We than compared these datasets to already existing datasets of other metrondiazole resistant lines and managed to identify a few core changes which are shared between all lines. In all lines proteins that are activating the drug are downregulated while proteins that are part of the oxidative stress response are found in higher abundance. On the other side these core changes are accompanied by a line specific set of changes, showing that there is more than one way for Giardia intestinalis to become resistant to metronidazole.
HostingRepository	PRIDE
AnnounceDate	2022-01-26
AnnouncementXML	Submission_2022-01-26_02:43:54.857.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027813
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sascha Krakovka
SpeciesList	scientific name: Giardia lamblia ATCC 50803; NCBI TaxID: 184922;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-09 06:34:04	ID requested
⏵ 1	2022-01-26 02:43:55	announced

Dataset with its publication pending

submitter keyword: Giardia intestinalis, metronidazole resistance, LC-MS

Staffan Svärd
contact affiliation	Department of Cell and Molecular Biology, Microbiology and Immunology, Uppsala University, Sweden
contact email	staffan.svard@icm.uu.se
lab head
Sascha Krakovka
contact affiliation	Uppsala University
contact email	sascha.krakovka@icm.uu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD027813

PRIDE project URI

• PRIDE
  1. PXD027813
     1. Label: PRIDE project
     2. Name: Comparative LC-MS analysis of two metronidazole resistant (M1,M2) and one revertant (M1NR) Giardia intestinalis strains with their parental strain (WB-C6)