PXD027799

PXD027799 is an original dataset announced via ProteomeXchange.

Title	Reversible Click Chemistry Tag for Universal Proteome Sample Preparation
Description	Here, we introduce a reversible protein tag, ProMTag, that enables whole proteome capture, cleanup, and release of intact proteins for top-down analysis. Alternatively, the addition of a novel Trypsin derivative to the workflow generates peptides for bottom-up analysis. We show that the ProMTag workflow yields >90% for intact proteins and >85% for proteome digests. For top-down analysis, ProMTag cleanup improves resolution on 2D gels; for bottom-up exploration, this methodology produced reproducible mass spectrometry results, demonstrating that the ProMTag method is a truly universal approach that produces high quality proteome samples compatible with multiple downstream analytical techniques.
HostingRepository	PRIDE
AnnounceDate	2022-02-14
AnnouncementXML	Submission_2022-02-14_02:19:01.982.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonathan Minden
SpeciesList	scientific name: Burkholderia sp. 2332; NCBI TaxID: 1219690; scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-09 06:32:39	ID requested
⏵ 1	2022-02-14 02:19:02	announced

Biedka S, Schmidt BF, Frey NM, Boothman SM, Minden JS, Wilson AL, Reversible Click Chemistry Tag for Universal Proteome Sample Preparation for Top-Down and Bottom-Up Analysis. J Proteome Res, 20(10):4787-4800(2021) [pubmed]

submitter keyword: Sample Preparation, Click chemistry, proteomics

Amber Lucas
contact affiliation	Impact Proteomics LLC, Pittsburgh, PA, USA 15206
contact email	amber.lucas@impactproteomics.com
lab head
Jonathan Minden
contact affiliation	Carnegie Mellon University
contact email	minden@cmu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027799

PRIDE project URI

• PRIDE
  1. PXD027799
     1. Label: PRIDE project
     2. Name: Reversible Click Chemistry Tag for Universal Proteome Sample Preparation