PXD027792

PXD027792 is an original dataset announced via ProteomeXchange.

Title	Proteins interacting with ZC3H28 and DRBD18
Description	ZC3H28 is a zinc-finger domain protein that is essential in bloodstream forms trypanosomes. Tethering ZC3H28 to a reporter mRNA results in an increase of the reporter mRNA levels but without a corresponding increase in protein expression levels. ZC3H28 associates with long RNAs that have low complexity in their 3’-untranslated regions. RNA immunoprecipitation results also showed that there was also a clear negative correlation between ZC3H28 mRNA binding and ribosome density. DRBD18 is a double RNA-binding domain protein that is essential in bloodstream forms and in procyclic forms. RNAi-mediated depletion of DRBD18 in bloodstream forms leads to reduction in RBP10 protein expression levels and caused accumulatio of several shortened RBP10 mRNA isoforms. DRBD18 depletion had similar effects on more than other 100 mRNAs including DRBD12. Binding of DRBD18 to mRNAs did not correlate with effects on the transcriptome, presumably because binding to mRNA precursors is not detected. We speculate that DRBD18 might bind to processing signals in the 3'-UTRs of mRNAs, thus preventing their use, and that this binding also serves to recruit mRNA export factors.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:17:36.261.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tania Bishola
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-06 07:20:15	ID requested
1	2022-10-14 07:29:19	announced
⏵ 2	2023-11-14 08:17:37	announced	2023-11-14: Updated project metadata.

Bishola Tshitenge T, Clayton C, splicing and polyadenylation patterns. RNA, 28(9):1239-1262(2022) [pubmed]

submitter keyword: RNA-binding proteins

Trypanosoma brucei

post-transcriptional regulation

Christine Clayton
contact affiliation	ZMBH, University of Heidelberg
contact email	cclayton@zmbh.uni-heidelberg.de
lab head
Tania Bishola
contact affiliation	ZMBH, University of Heidelberg
contact email	taniabishola@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD027792

PRIDE project URI

• PRIDE
  1. PXD027792
     1. Label: PRIDE project
     2. Name: Proteins interacting with ZC3H28 and DRBD18