PXD027784

PXD027784 is an original dataset announced via ProteomeXchange.

Title	A proteomic approach for unravelling the relevance of the polyadenylation factor EhCFIm25 in Entamoeba histolytica survival and virulence
Description	Please provide an overall description of your study, think something similar in scope to the manuscript abstractCFIm25 is essential for recruitment of other polyadenylation factors, poly(A) site selection, pre-mRNA cleavage and poly(A) tail synthesis. We recently reported that EhCFIm25 silencing in Entamoeba histolytica, the protozoan responsible for amebic dysentery, affects trophozoite proliferation and death, and reduces parasite virulence, indicating its relevance as a new biochemical target in this human pathogen. Here, we performed a shotgun proteomic analysis to evidence the impact of EhCFIm25 silencing on protein expression that can explain the observed phenotype
HostingRepository	PRIDE
AnnounceDate	2021-08-26
AnnouncementXML	Submission_2021-08-26_04:00:04.831.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027784
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rodolfo G Avila-Bonilla
SpeciesList	scientific name: Entamoeba histolytica HM-1:IMSS-A; NCBI TaxID: 885318;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt G2 MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-06 03:45:18	ID requested
⏵ 1	2021-08-26 04:00:05	announced

Dataset with its publication pending

submitter keyword: i.e. Human, Liver, Plntamoeba histolytica

CFIm25 silencing

polyadenylation

proteomics

virulenceasma, LC-MSMS

Laurence A. Marchat
contact affiliation	Instituto Politécnico Nacional
contact email	lmarchat@gmail.com
lab head
Rodolfo G Avila-Bonilla
contact affiliation	Instituto Politécnico Nacional
contact email	avilab.r.g@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027784
     1. Label: PRIDE project
     2. Name: A proteomic approach for unravelling the relevance of the polyadenylation factor EhCFIm25 in Entamoeba histolytica survival and virulence