PXD027783

PXD027783 is an original dataset announced via ProteomeXchange.

Title	IDA (Iminodiacetic Acid) Generated Mesoporous Nanopolymer - A Template to Relate Surface Area, Hydrophilicity and Glycopeptides Enrichment
Description	Co-polymer 1,2-epoxy-5-hexene and divinyl benzene with terminal oxirane group is synthesized for the first time. High density of hydrophilic groups (-COOH, -OH) enhances hydrophilicity through facile functionalization with Diethylene Triamine (DETA) and chloroacetic acid. COOH-functionalized mesoporous polymeric beads provide high surface area and porosity, superior hydrophilicity, solvent resistance and good bio-capability for enriching N-Linked glycopeptides. Sensitivity, selectivity, reusability and batch to batch reproducibility are tested using model glycoproteins (HRP, IgG and avidin) and analyzed by MALDI-TOF-MS. Furthermore, N-glycosylation sites in N-glycopeptides of characteristic glycoproteins are identified from digested human serum.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:02:29.867.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Muhammad Sajid
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	ultraflex; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-06 03:45:15	ID requested
1	2023-07-20 10:43:42	announced
⏵ 2	2023-11-14 09:02:33	announced	2023-11-14: Updated project metadata.

Sajid MS, Saleem S, Jabeen F, Fatima B, Zulfikar M, Ashiq MN, Ressom HW, Pukala TL, Najam-Ul-Haq M, Iminodiacetic acid (IDA)-generated mesoporous nanopolymer: a template to relate surface area, hydrophilicity, and glycopeptides enrichment. Mikrochim Acta, 188(12):417(2021) [pubmed]

submitter keyword: LC-MS/MS, SPE, polymer, glycopeptides, enrichment

Habtom W. Ressom
contact affiliation	Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.
contact email	hwr@georgetown.edu
lab head
Muhammad Sajid
contact affiliation	Georgetown University
contact email	MS4868@georgetown.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD027783

PRIDE project URI

• PRIDE
  1. PXD027783
     1. Label: PRIDE project
     2. Name: IDA (Iminodiacetic Acid) Generated Mesoporous Nanopolymer - A Template to Relate Surface Area, Hydrophilicity and Glycopeptides Enrichment