PXD027781

PXD027781 is an original dataset announced via ProteomeXchange.

Title	Voltage-Dependent Anion-Selective Channels and Other Mitochondrial Membrane Proteins Form Diverse Complexes in Beetroots Subjected to Flood-Induced Programmed Cell Death
Description	In plants, programmed cell death (PCD) is involved in both the development and in the response to biotic and abiotic aggressions. In early stages of PCD, mitochondrial membranes are made permeable by the formation of permeability transition pores, whose protein composition is debated. Since flooding stress can produce PCD in several plant species, the first goal of this work was to know if flooding stress could be used to induce PCD in Beta vulgaris roots. To do this, two months old beet plants were flood-stressed from one to five days, and the alterations indicating PCD in stressed beetroot cells were observed with a confocal fluorescence microscope. In addition, cytochrome c was released from mitochondria. After assessing that flood stress induced PCD in beetroots, the composition of mitochondrial protein complexes was observed in control and flood-stressed beetroots. Protein complexes from isolated mitochondria were separated by native gel electrophoresis, and their proteins were identified by mass spectrometry. The spectra count of three isoforms of voltage-dependent anion-selective channels (VDAC) increased after one day of flooding. In addition, the size of the complexes formed by VDAC was higher in beetroots flood-stressed for one day (~200 kDa) compared with non-stressed ones (~100 kDa). Other proteins, like chaperonin CPN60-2, also formed complexes with different masses in control and flood-stressed beetroots. Finally, possible interactions of VDAC with other proteins were found performing a cluster analysis. These results indicate that mitochondrial protein complexes formed by VDAC could be involved in the process of programmed cell death in flood-stressed beetroots
HostingRepository	PRIDE
AnnounceDate	2021-10-28
AnnouncementXML	Submission_2021-10-28_01:38:42.888.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	KARLA JOANNA ROJAS MENDEZ
SpeciesList	scientific name: Beta vulgaris subsp. vulgaris; NCBI TaxID: 3555;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-06 03:45:06	ID requested
⏵ 1	2021-10-28 01:38:44	announced

Rojas-M, é, ndez KJ, S, á, nchez Segura L, Chagolla A, Lino B, Gonz, á, lez de la Vara LE, Voltage-Dependent Anion-Selective Channels and Other Mitochondrial Membrane Proteins Form Diverse Complexes in Beetroots Subjected to Flood-Induced Programmed Cell Death. Front Plant Sci, 12():714847(2021) [pubmed]

submitter keyword: Beta vulgaris, Flooding stress, Mitochondrial membrane protein complexes, Programmed cell death (PCD), Voltage-dependent anion-selective channels (VDAC)

Luis E. Gonzalez de la Vara
contact affiliation	Department of Biotechnology and Biochemistry, Bioenergetics and biomembranes laboratory, Centro de Investigación y Estudios Avanzados del IPN - Unidad Irapuato, México
contact email	luis.gonzalezd@cinvestav.mx
lab head
KARLA JOANNA ROJAS MENDEZ
contact affiliation	Cinvestav
contact email	karla.rojas@cinvestav.mx
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027781
     1. Label: PRIDE project
     2. Name: Voltage-Dependent Anion-Selective Channels and Other Mitochondrial Membrane Proteins Form Diverse Complexes in Beetroots Subjected to Flood-Induced Programmed Cell Death