PXD027781 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Voltage-Dependent Anion-Selective Channels and Other Mitochondrial Membrane Proteins Form Diverse Complexes in Beetroots Subjected to Flood-Induced Programmed Cell Death |
Description | In plants, programmed cell death (PCD) is involved in both the development and in the response to biotic and abiotic aggressions. In early stages of PCD, mitochondrial membranes are made permeable by the formation of permeability transition pores, whose protein composition is debated. Since flooding stress can produce PCD in several plant species, the first goal of this work was to know if flooding stress could be used to induce PCD in Beta vulgaris roots. To do this, two months old beet plants were flood-stressed from one to five days, and the alterations indicating PCD in stressed beetroot cells were observed with a confocal fluorescence microscope. In addition, cytochrome c was released from mitochondria. After assessing that flood stress induced PCD in beetroots, the composition of mitochondrial protein complexes was observed in control and flood-stressed beetroots. Protein complexes from isolated mitochondria were separated by native gel electrophoresis, and their proteins were identified by mass spectrometry. The spectra count of three isoforms of voltage-dependent anion-selective channels (VDAC) increased after one day of flooding. In addition, the size of the complexes formed by VDAC was higher in beetroots flood-stressed for one day (~200 kDa) compared with non-stressed ones (~100 kDa). Other proteins, like chaperonin CPN60-2, also formed complexes with different masses in control and flood-stressed beetroots. Finally, possible interactions of VDAC with other proteins were found performing a cluster analysis. These results indicate that mitochondrial protein complexes formed by VDAC could be involved in the process of programmed cell death in flood-stressed beetroots |
HostingRepository | PRIDE |
AnnounceDate | 2021-10-28 |
AnnouncementXML | Submission_2021-10-28_01:38:42.888.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | KARLA JOANNA ROJAS MENDEZ |
SpeciesList | scientific name: Beta vulgaris subsp. vulgaris; NCBI TaxID: 3555; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-08-06 03:45:06 | ID requested | |
⏵ 1 | 2021-10-28 01:38:44 | announced | |
Publication List
Rojas-M, é, ndez KJ, S, á, nchez Segura L, Chagolla A, Lino B, Gonz, á, lez de la Vara LE, Voltage-Dependent Anion-Selective Channels and Other Mitochondrial Membrane Proteins Form Diverse Complexes in Beetroots Subjected to Flood-Induced Programmed Cell Death. Front Plant Sci, 12():714847(2021) [pubmed] |
Keyword List
submitter keyword: Beta vulgaris, Flooding stress, Mitochondrial membrane protein complexes, Programmed cell death (PCD), Voltage-dependent anion-selective channels (VDAC) |
Contact List
Luis E. Gonzalez de la Vara |
contact affiliation | Department of Biotechnology and Biochemistry, Bioenergetics and biomembranes laboratory, Centro de Investigación y Estudios Avanzados del IPN - Unidad Irapuato, México |
contact email | luis.gonzalezd@cinvestav.mx |
lab head | |
KARLA JOANNA ROJAS MENDEZ |
contact affiliation | Cinvestav |
contact email | karla.rojas@cinvestav.mx |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027781
- Label: PRIDE project
- Name: Voltage-Dependent Anion-Selective Channels and Other Mitochondrial Membrane Proteins Form Diverse Complexes in Beetroots Subjected to Flood-Induced Programmed Cell Death