⮝ Full datasets listing

PXD027781

PXD027781 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleVoltage-Dependent Anion-Selective Channels and Other Mitochondrial Membrane Proteins Form Diverse Complexes in Beetroots Subjected to Flood-Induced Programmed Cell Death
DescriptionIn plants, programmed cell death (PCD) is involved in both the development and in the response to biotic and abiotic aggressions. In early stages of PCD, mitochondrial membranes are made permeable by the formation of permeability transition pores, whose protein composition is debated. Since flooding stress can produce PCD in several plant species, the first goal of this work was to know if flooding stress could be used to induce PCD in Beta vulgaris roots. To do this, two months old beet plants were flood-stressed from one to five days, and the alterations indicating PCD in stressed beetroot cells were observed with a confocal fluorescence microscope. In addition, cytochrome c was released from mitochondria. After assessing that flood stress induced PCD in beetroots, the composition of mitochondrial protein complexes was observed in control and flood-stressed beetroots. Protein complexes from isolated mitochondria were separated by native gel electrophoresis, and their proteins were identified by mass spectrometry. The spectra count of three isoforms of voltage-dependent anion-selective channels (VDAC) increased after one day of flooding. In addition, the size of the complexes formed by VDAC was higher in beetroots flood-stressed for one day (~200 kDa) compared with non-stressed ones (~100 kDa). Other proteins, like chaperonin CPN60-2, also formed complexes with different masses in control and flood-stressed beetroots. Finally, possible interactions of VDAC with other proteins were found performing a cluster analysis. These results indicate that mitochondrial protein complexes formed by VDAC could be involved in the process of programmed cell death in flood-stressed beetroots
HostingRepositoryPRIDE
AnnounceDate2021-10-28
AnnouncementXMLSubmission_2021-10-28_01:38:42.888.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKARLA JOANNA ROJAS MENDEZ
SpeciesList scientific name: Beta vulgaris subsp. vulgaris; NCBI TaxID: 3555;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-08-06 03:45:06ID requested
12021-10-28 01:38:44announced
Publication List
Rojas-M, é, ndez KJ, S, á, nchez Segura L, Chagolla A, Lino B, Gonz, á, lez de la Vara LE, Voltage-Dependent Anion-Selective Channels and Other Mitochondrial Membrane Proteins Form Diverse Complexes in Beetroots Subjected to Flood-Induced Programmed Cell Death. Front Plant Sci, 12():714847(2021) [pubmed]
Keyword List
submitter keyword: Beta vulgaris, Flooding stress, Mitochondrial membrane protein complexes, Programmed cell death (PCD), Voltage-dependent anion-selective channels (VDAC)
Contact List
Luis E. Gonzalez de la Vara
contact affiliationDepartment of Biotechnology and Biochemistry, Bioenergetics and biomembranes laboratory, Centro de Investigación y Estudios Avanzados del IPN - Unidad Irapuato, México
contact emailluis.gonzalezd@cinvestav.mx
lab head
KARLA JOANNA ROJAS MENDEZ
contact affiliationCinvestav
contact emailkarla.rojas@cinvestav.mx
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/10/PXD027781
PRIDE project URI
Repository Record List
[ + ]