PXD027777

PXD027777 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Downstream targets of Ca2+ signals in filamentous fungi
Description	Ca2+ is a second messenger that regulates a variety of cellular responses in fungi. However, the mechanism behind how Ca2+ signals are transmitted within the cell as they regulate downstream targets remains unclear. We used a fluorescent Ca2+ biosensor to visualize the changes in Ca2+ concentration in the hyphae of the filamentous fungus Aspergillus nidulans in response to thermal stress via infrared laser point irradiation, and then we analyzed the characteristics of the periodic Ca2+ influx in the hyphae. Stress-responsive Ca2+ pulses promoted the reorganization of the actin cytoskeleton and exocytosis, and they controlled the resumption, redirection, or branching of hyphal growth. To clarify how calcium signals are transmitted to downstream targets, we focused on calmodulin (CaM) and calcium/CaM-dependent protein kinases (CaMKs; CmkA, CmkB, and CmkC) by clarifying their localization and studying their interacting proteins by GFP-trap and MS analysis. Analysis of proteins that co-precipitated with CaM or CamKs showed that the calcium signaling pathway is involved in actin cytoskeleton rearrangement, vesicle transport, cell wall synthesis, and the cell wall integrity (CWI) pathway. Our approach enabled us to visualize the actual conduction of Ca2+ in hyphae in response to stress, and it clarified the downstream targets of CaM and CaMKs, thereby connecting the series of events, from input to output that are involved in calcium signaling.
HostingRepository	jPOST
AnnounceDate	2022-09-08
AnnouncementXML	Submission_2022-09-18_03:21:25.005.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shunsuke Masuo
SpeciesList	scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationList	S-carboxamidomethyl-L-cysteine; N6-acetyl-L-lysine; L-methionine sulfoxide
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-06 00:22:53	ID requested
1	2022-09-07 08:00:04	announced
⏵ 2	2022-09-18 03:21:25	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Aspergillus nidulans, calmodulin-dependent kinases, LC-MSMS

submitter keyword: Aspergillus nidulans, calmodulin-dependent kinases, LC-MSMS

Contact List

Norio Takeshita
lab head
Shunsuke Masuo
contact affiliation	University of Tsukuba
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001285/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001285/