PXD027774

PXD027774 is an original dataset announced via ProteomeXchange.

Title	Hydra vulgaris extracellular vesicles
Description	Recent body of evidence demonstrates that extracellular vesicles (EVs) represent the first language of cell-cell communication emerged during evolution. In aquatic environments, transferring signals between cells by EVs offer protection against degradation, allowing delivering of chemical information in high local concentrations to the target cells. The packaging of multiple signals, including those of hydrophobic nature, ensures target cells to receive the same EV-conveyed messages, and the coordination of a variety of physiological processes across cells of a single organisms, or at the population level, i.e. mediating the population´s response to changing environmental conditions. Here, we purified EVs from the medium of the freshwater invertebrate Hydra vulgaris, and the molecular profiling by proteomic and transcriptomic analyses revealed multiple markers of the exosome EV subtype. Moreover, positive and negative regulators of the Wnt/β-catenin signaling pathway, the major developmental pathway acting in body axial patterning, were identified. Functional analysis on amputated polyps revealed EV ability to interfere with both head and foot regeneration, suggesting an active role in setting up tissue gradients and oro-aboral polarity through delivery of short and long-distance signals. Our results open the path to unravel EV biogenesis and function in all cnidarian species, tracing back the origin of the cell-cell, cross-species or cross-kingdom communication in aquatic ecosystems
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_04:15:46.022.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Moros
SpeciesList	scientific name: Hydra vulgaris; NCBI TaxID: 6087;
ModificationList	No PTMs are included in the dataset
Instrument	Q TRAP

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-05 10:12:34	ID requested
⏵ 1	2022-02-17 04:15:46	announced

Moros M, Fergola E, Marchesano V, Mutarelli M, Tommasini G, Miedziak B, Palumbo G, Ambrosone A, Tino A, Tortiglione C, Releases Molecular Messages Through Extracellular Vesicles. Front Cell Dev Biol, 9():788117(2021) [pubmed]

submitter keyword: Hydra vulgaris, extracellular vesicles, LC-ESI-MS/MS

Maria Moros
contact affiliation	Investigador Ramon y Cajal, grupo Bionanosurf, Instituto de nanociencia y materiales de ARagón (Zaragoza, España)
contact email	mamoros@unizar.es
lab head
Maria Moros
contact affiliation	Instituto de Nanociencia y Materiales de ARagón
contact email	mamoros@unizar.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027774

PRIDE project URI

• PRIDE
  1. PXD027774
     1. Label: PRIDE project
     2. Name: Hydra vulgaris extracellular vesicles