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PXD027769 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSystematic investigation of protein phosphorylation in biomolecular condensates
DescriptionWe have adapted a recently developed quantitative proteomics based approach to measure protein solubility following a lysate centrifugation assay to determine the proportion of a protein in distinct subpopulations in a proteome-wide manner (Sridharan et al., 2019). Further, by combining the solubility measurements with phosphoproteomics (Potel et al., 2021), we have quantitatively mapped phosphorylation sites enriched in the different protein subpopulations.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSindhuja Sridharan
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-08-05 10:02:45ID requested
12022-08-09 06:57:11announced
Publication List
Sridharan S, Hernandez-Armendariz A, Kurzawa N, Potel CM, Memon D, Beltrao P, Bantscheff M, Huber W, Cuylen-Haering S, Savitski MM, Systematic discovery of biomolecular condensate-specific protein phosphorylation. Nat Chem Biol, 18(10):1104-1114(2022) [pubmed]
Keyword List
submitter keyword: protein solubility, phosphorylation, biomolecular condensate
Contact List
Mikhail M. Savitski
contact affiliationEuropean Molecular Biology Laboratory
contact emailmikhail.savitski@embl.de
lab head
Sindhuja Sridharan
contact affiliationEuropean Molecular Biology Laboratory
contact emailsindhuja.sridharan@embl.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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