PXD027769

PXD027769 is an original dataset announced via ProteomeXchange.

Title	Systematic investigation of protein phosphorylation in biomolecular condensates
Description	We have adapted a recently developed quantitative proteomics based approach to measure protein solubility following a lysate centrifugation assay to determine the proportion of a protein in distinct subpopulations in a proteome-wide manner (Sridharan et al., 2019). Further, by combining the solubility measurements with phosphoproteomics (Potel et al., 2021), we have quantitatively mapped phosphorylation sites enriched in the different protein subpopulations.
HostingRepository	PRIDE
AnnounceDate	2022-08-09
AnnouncementXML	Submission_2022-08-09_06:57:10.531.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sindhuja Sridharan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-05 10:02:45	ID requested
⏵ 1	2022-08-09 06:57:11	announced

Sridharan S, Hernandez-Armendariz A, Kurzawa N, Potel CM, Memon D, Beltrao P, Bantscheff M, Huber W, Cuylen-Haering S, Savitski MM, Systematic discovery of biomolecular condensate-specific protein phosphorylation. Nat Chem Biol, 18(10):1104-1114(2022) [pubmed]

submitter keyword: protein solubility, phosphorylation, biomolecular condensate

Mikhail M. Savitski
contact affiliation	European Molecular Biology Laboratory
contact email	mikhail.savitski@embl.de
lab head
Sindhuja Sridharan
contact affiliation	European Molecular Biology Laboratory
contact email	sindhuja.sridharan@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD027769

PRIDE project URI

• PRIDE
  1. PXD027769
     1. Label: PRIDE project
     2. Name: Systematic investigation of protein phosphorylation in biomolecular condensates