PXD027766 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mapping the soluble human leukocyte antigen peptidome of pleural effusions reveals lung and tumor-associated antigens |
Description | Soluble HLA (sHLA) molecules released to the plasma, carry their original peptide cargo and provide insight into the protein synthesis and degradation schemes of their source cells and tissues. Other body fluids, such as pleural effusions, may also contain sHLA-peptide complexes, and can potentially serve as a source of tumor antigens since these fluids are drained from the tumor microenvironment. Thus, we developed a methodology for purifying and analysing large pleural effusion sHLA class I peptidomes of patients inflicted with malignancies or benign diseases. The cleared pleural fluids, the cell pellets present in the pleural effusions, and the primary tumor cells cultured from cancer patients’ effusions, were used for immunoaffinity purification of the HLA molecules. The recovered HLA peptides were analyzed by capillary chromatography coupled to tandem mass spectrometry and the resulting LC-MS/MS data was analyzed with the MaxQuant software tool. Large HLA peptidomes were obtained by the analysis of the pleural effusions. The majority of peptides identified from the pleural effusions were defined as HLA ligands that fit the patients’ HLA consensus sequence motifs. The membranal and soluble HLA peptidomes of each individual patient were somewhat similar to each other. Many of the HLA peptides were derived from known tumor-associated antigens, lung-related proteins, and VEGF pathway proteins. Thus, the pleural effusion HLA peptidome of patients with malignant tumors can serve as a rich source of biomarkers for tumor diagnosis and personalized immunotherapy. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-25 |
AnnouncementXML | Submission_2022-05-25_04:14:39.906.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dganit Melamed Kadosh |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-08-05 10:02:30 | ID requested | |
⏵ 1 | 2022-05-25 04:14:40 | announced | |
Publication List
Khazan-Kost S, Cafri G, Melamed Kadosh D, Mooshayef N, Chatterji S, Dominissini D, Manor S, Zisser B, Broday L, Talalai E, Shemer A, Zadok O, Ofek E, Onn A, Admon A, Peled M, Soluble HLA peptidome of pleural effusions is a valuable source for tumor antigens. J Immunother Cancer, 10(5):(2022) [pubmed] |
Keyword List
submitter keyword: Pleural effusion, HLA peptidome, immunopeptisome, LC-MS/MS, lung cancer, tumor antigens, cancer/testis antigens |
Contact List
Arie Admon |
contact affiliation | Faculty of Biology, Technion-Israel Institute of Technology, Haifa, Israel |
contact email | admon@technion.ac.il |
lab head | |
Dganit Melamed Kadosh |
contact affiliation | Technion |
contact email | sdganit@tx.technion.ac.il |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027766
- Label: PRIDE project
- Name: Mapping the soluble human leukocyte antigen peptidome of pleural effusions reveals lung and tumor-associated antigens