PXD027753

PXD027753 is an original dataset announced via ProteomeXchange.

Title	Identification of allergenomic signatures in allergic and well-tolerated apple genotypes using LC-MS/MS
Description	The apple fruit (Malus domestica L. Borkh) is one of the most popular fruits worldwide. Beyond their beneficial properties, apples contain proteins that trigger allergic reactions in susceptible consumers. Mal d1 to 4 are allergens present in a variety of different isoforms in apples, with Mal d1 and Mal d3 being the main allergens. In this study, we used proteomics to quantify all four Mal d proteins in 52 apple genotypes with varying allergenic potentials. We revealed the expression of at least 21 Mal d1 isoforms while two Mal d3 and two Mal d4 proteins were found among the genotypes. The allergenic potential of the Mal d isoforms was characterized by comparing isoform abundance with allergenic scores of genotypes (from oral challenge tests). The Mal d peptides detected by comparative analysis presumably have different IgE binding properties and could therefore be used as potential molecular markers to discriminate between hypoallergenic and hyperallergenic cultivars.
HostingRepository	PRIDE
AnnounceDate	2022-05-24
AnnouncementXML	Submission_2022-05-24_08:00:50.525.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christina Ludwig
SpeciesList	scientific name: Malus domestica; NCBI TaxID: 3750;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-05 10:00:57	ID requested
⏵ 1	2022-05-24 08:00:51	announced

Chebib S, Meng C, Ludwig C, Bergmann KC, Becker S, Dierend W, Schwab W, Identification of allergenomic signatures in allergic and well-tolerated apple genotypes using LC-MS/MS. Food Chem (Oxf), 4():100111(2022) [pubmed]

submitter keyword: Malus domestica, Mal d proteins, proteomics, LC-MS/MS, hypoallergenic, apple breeding

Dr. Christina Ludwig
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS), Technical University Munich, Gregor-Mendel-Straße 4, 85354, Freising, Germany
contact email	tina.ludwig@tum.de
lab head
Christina Ludwig
contact affiliation	TU Munich
contact email	tina.ludwig@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD027753

PRIDE project URI

• PRIDE
  1. PXD027753
     1. Label: PRIDE project
     2. Name: Identification of allergenomic signatures in allergic and well-tolerated apple genotypes using LC-MS/MS