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PXD027739 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRelease of a nuclear ubiquitin-like protein activates proteasomal degradation of mislocalized hydrophobic mitochondrial proteins in trypanosomes
DescriptionMitochondrial protein import in the parasitic protozoan Trypanosoma brucei is mediated by the atypical outer membrane translocase, ATOM. It consists of seven subunits including ATOM69, the import receptor for hydrophobic proteins. Ablation of ATOM69, but not of any other subunit, triggers a unique quality control pathway resulting in the proteasomal degradation of non-imported mitochondrial proteins. The process requires a protein of unknown function, an E3 ubiquitin ligase and the ubiquitin-like protein (TbUbL1), which all are recruited to the mitochondrion upon ATOM69 depletion. TbUbL1 is a nuclear protein, a fraction of which is released to the cytosol upon triggering of the pathway. Nuclear release is essential since cytosolic TbUbL1 can bind mislocalized mitochondrial proteins and likely transfers them to the proteasome. Mitochondrial quality control has previously been studied in yeast and metazoans. Finding such a pathway in the highly diverged trypanosomes suggests such pathways are an obligate feature of all mitochondria.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFriedel Drepper
SpeciesList scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-08-05 08:59:49ID requested
12022-05-04 04:50:58announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: SILAC, RNAi-mediated protein knockdown, proteomics, Trypanosoma brucei, mitochondrial quality control
Contact List
Bettina Warscheid
contact affiliationUniversity of Freiburg, Institute of Biology II, Biochemistry/Functional Proteomics
contact emailbettina.warscheid@biologie.uni-freiburg.de
lab head
Friedel Drepper
contact affiliationAG Warscheid Biologie II Albert-Ludwigs-Universität Freiburg Schänzlestr. 1 79104 Freiburg Germany
contact emailfriedel.drepper@biologie.uni-freiburg.de
dataset submitter
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Dataset FTP location
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