PXD027735

PXD027735 is an original dataset announced via ProteomeXchange.

Title	MS proteomic datasets for "Development of methodology to investigate the surface SMALPome of mammalian cells".
Description	In this study, mass spectrometry proteomics was used to investigate whether there are qualitative or quantitative differences in the mammalian plasma membrane proteins extracted with SMA compared to a detergent control. For this, cell surface proteins of 3T3L1 fibroblasts were biotinylated and extracted using either styrene maleic acid (SMA) or detergent. Following affinity pull-down of biotinylated proteins with NeutrAvidin beads, samples were analysed by nanoLC-MS. Here, we report for the first time, a global proteomics protocol for detection of a mammalian cell ‘SMALPome’, membrane proteins incorporated into SMA nanodiscs.
HostingRepository	PRIDE
AnnounceDate	2021-12-15
AnnouncementXML	Submission_2021-12-15_02:38:23.101.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kerrie Morrison
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	biotinylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-05 08:59:28	ID requested
⏵ 1	2021-12-15 02:38:23	announced

Dataset with its publication pending

submitter keyword: mouse, 3T3L1 fibroblasts, nano-LC-MS

Paul Whitley
contact affiliation	Department of Biology and Biochemistry University of Bath Bath UK
contact email	bssprw@bath.ac.uk
lab head
Kerrie Morrison
contact affiliation	University of Bath
contact email	kam84@bath.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD027735

PRIDE project URI

• PRIDE
  1. PXD027735
     1. Label: PRIDE project
     2. Name: MS proteomic datasets for "Development of methodology to investigate the surface SMALPome of mammalian cells".