PXD027723

PXD027723 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometric and bio-computational analysis of multiply charged RNase S gas phase complexes obtained by electrospray ionization from varying in-solution equilibrium conditions
Description	In this project we investigate the influence of a solvent's composition on the stability of desorbed and multiply charged RNase S ions by analyzing gas phase dissociation reactions triggered by collision induced dissociation (CID) of multiply charged complex ions. RNase S was dissolved in ESI-compatible buffers with either an increasing content of organic co-solvent or with different pH. Direct transition of all ions from the in-solution components is followed by CID of the non-covalent RNase S complex and by quantitative analysis of the dissociation products. From normalized ion abundances are determined the apparent kinetic and apparent thermodynamic gas phase complex properties. The stability of RNase S in the gas phase is independent from the in-solution equilibrium but sensitive to differences in charge states.
HostingRepository	PRIDE
AnnounceDate	2021-11-23
AnnouncementXML	Submission_2021-11-23_03:49:43.809.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Kreutzer
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	Waters instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-05 08:58:08	ID requested
⏵ 1	2021-11-23 03:49:44	announced

Koy C, Opuni KFM, Danquah BD, Neamtu A, Glocker MO, Mass Spectrometric and Bio-Computational Binding Strength Analysis of Multiply Charged RNAse S Gas-Phase Complexes Obtained by Electrospray Ionization from Varying In-Solution Equilibrium Conditions. Int J Mol Sci, 22(19):(2021) [pubmed]

submitter keyword: Off-line ESI-MS, ITEM-TWO, RNase S, complex stability, varying content of MeOH co-solvent, different pH

Prof. Dr. Michael O. Glocker
contact affiliation	Proteome Center Rostock, University of Rostock, Germany
contact email	michael.glocker@med.uni-rostock.de
lab head
Michael Kreutzer
contact affiliation	University Medical Center Rostock
contact email	michael.kreutzer@med.uni-rostock.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD027723

PRIDE project URI

• PRIDE
  1. PXD027723
     1. Label: PRIDE project
     2. Name: Mass spectrometric and bio-computational analysis of multiply charged RNase S gas phase complexes obtained by electrospray ionization from varying in-solution equilibrium conditions