PXD027721

PXD027721 is an original dataset announced via ProteomeXchange.

Title	We applied an iodoacetyl tandem mass tags (iodoTMT)-based proteomic approach to analyze the redox proteome of Brassica napus seedlings under control and salt-stressed conditions. We identified 1,821 sulfenylated sites in 912 proteins from all samples.
Description	By means of large-scale redox proteomics, we studied reversible cysteine modification during the response to short-term salt stress in Brassica napus. We applied an iodoacetyl tandem mass tags (iodoTMT)-based proteomic approach to analyze the redox proteome of Brassica napus seedlings under control and salt-stressed conditions. We identified 1,821 sulfenylated sites in 912 proteins from all samples. A great number of sulfenylated proteins were predicted to localize to chloroplasts and cytoplasm and GO enrichment analysis of differentially sulfenylated proteins revealed that metabolic processes such as photosynthesis and glycolysis are enriched and enzymes are overrepresented. Redox-sensitive sites in two enzymes were validated in vitro on recombinant proteins and they might affect the enzyme activity. This targeted approach contributes to the identification of the sulfenylated sites and proteins in Brassica napus subjected to salt stress and our study will improve our understanding of the molecular mechanisms underlying the redox regulation in response to salt stress.
HostingRepository	PRIDE
AnnounceDate	2021-08-18
AnnouncementXML	Submission_2021-08-18_06:06:26.023.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liangqian Yu
SpeciesList	scientific name: Brassica napus (Rape); NCBI TaxID: 3708;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-05 05:52:52	ID requested
1	2021-08-18 05:58:39	announced
⏵ 2	2021-08-18 06:06:27	announced	2021-08-18: Updated publication reference for PubMed record(s): 34347306.

Yu L, Iqbal S, Zhang Y, Zhang G, Ali U, Lu S, Yao X, Guo L, Proteome-wide identification of S-sulphenylated cysteines in Brassica napus. Plant Cell Environ, 44(11):3571-3582(2021) [pubmed]

submitter keyword: Brassica napus, leaf, LC-MS/MS

Liang Guo
contact affiliation	National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University
contact email	guoliang@mail.hzau.edu.cn
lab head
Liangqian Yu
contact affiliation	1534447256@qq.com
contact email	1534447256@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027721
     1. Label: PRIDE project
     2. Name: We applied an iodoacetyl tandem mass tags (iodoTMT)-based proteomic approach to analyze the redox proteome of Brassica napus seedlings under control and salt-stressed conditions. We identified 1,821 sulfenylated sites in 912 proteins from all samples.