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PXD027719

PXD027719 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLC-MS and gel-based comparison of DPSCs and PDLSCs
DescriptionTooth pulp contains various types of cells such as endothelial cells, neurons, fibroblasts, osteoblasts, osteoclasts, and odontoblasts. As well as cells that are called "postnatal dental pulp stem cells" (DPSCs). Also, four more types of dental MSC-like populations were identified and characterized: stem cells from human exfoliated deciduous teeth (SHED), periodontal ligament stem cells (PDLSCs), stem cells from the apical papilla (SCAP) and population of dental follicle-derived progenitor cells called "dental follicle progenitor cells" (DFPCs). Most of them might be used in wide range of biomedical applications. Nevertheless, they have systematic differences in their physiology, e.g. differences in proliferative and differentiation potential. These differences are not clearly defined on molecular level yet; therefore, we performed proteomics comparison of DPSCs and PDLSCs in control and osteogenic differentiation. Donor matched DPSCs and PDLSCs were isolated from two donors by standard protocol. Then, DPSCs and PDLSCs at passage 3 were seeded into 90 mm Petri dishes (Eppendorf) and cultured in standard conditions with DMEM (Gibco) supplemented with 15% fetal bovine serum (FBS), 37°C, 5% CO2. When cells reached 90-100% confluency, the medium was changed to osteogenic medium (DMEM supplemented with 10% FBS, 2 mM L-glutamine, 1% penicillin/streptomycin (HyClone), 50 mg/ml ascorbic acid (Sigma Aldrich), 0.1 mM dexamethasone (Sigma Aldrich) and 10 mM b–glycerophosphate (Sigma Aldrich).
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:32:54.227.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD027719
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterArseniy Lobov
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-08-05 05:47:54ID requested
12022-02-18 08:36:40announced
22024-10-22 05:32:54announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD027719;
Keyword List
submitter keyword: osteogenic differentiation,MSCs, TimsToF Pro, PDLSCs, 2D DIGE, DPSCs, ion mobility
Contact List
Natella Enukashvily
contact affiliationHead of the Laboratory of non-coding DNA, Institute of Cytology RAS, St. Petersburg, Russia
contact emailn.enukashvily@incras.ru
lab head
Arseniy Lobov
contact affiliationYifat Merbl Lab, Dept. of Systems Immunology, Weizmann Institute of Science
contact emailarseniylobov@gmail.com
dataset submitter
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