PXD027719

PXD027719 is an original dataset announced via ProteomeXchange.

Title	LC-MS and gel-based comparison of DPSCs and PDLSCs
Description	Tooth pulp contains various types of cells such as endothelial cells, neurons, fibroblasts, osteoblasts, osteoclasts, and odontoblasts. As well as cells that are called "postnatal dental pulp stem cells" (DPSCs). Also, four more types of dental MSC-like populations were identified and characterized: stem cells from human exfoliated deciduous teeth (SHED), periodontal ligament stem cells (PDLSCs), stem cells from the apical papilla (SCAP) and population of dental follicle-derived progenitor cells called "dental follicle progenitor cells" (DFPCs). Most of them might be used in wide range of biomedical applications. Nevertheless, they have systematic differences in their physiology, e.g. differences in proliferative and differentiation potential. These differences are not clearly defined on molecular level yet; therefore, we performed proteomics comparison of DPSCs and PDLSCs in control and osteogenic differentiation. Donor matched DPSCs and PDLSCs were isolated from two donors by standard protocol. Then, DPSCs and PDLSCs at passage 3 were seeded into 90 mm Petri dishes (Eppendorf) and cultured in standard conditions with DMEM (Gibco) supplemented with 15% fetal bovine serum (FBS), 37°C, 5% CO2. When cells reached 90-100% confluency, the medium was changed to osteogenic medium (DMEM supplemented with 10% FBS, 2 mM L-glutamine, 1% penicillin/streptomycin (HyClone), 50 mg/ml ascorbic acid (Sigma Aldrich), 0.1 mM dexamethasone (Sigma Aldrich) and 10 mM b–glycerophosphate (Sigma Aldrich).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:32:54.227.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027719
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Arseniy Lobov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-05 05:47:54	ID requested
1	2022-02-18 08:36:40	announced
⏵ 2	2024-10-22 05:32:54	announced	2024-10-22: Updated project metadata.

10.6019/PXD027719;

submitter keyword: osteogenic differentiation,MSCs, TimsToF Pro, PDLSCs, 2D DIGE, DPSCs, ion mobility

Natella Enukashvily
contact affiliation	Head of the Laboratory of non-coding DNA, Institute of Cytology RAS, St. Petersburg, Russia
contact email	n.enukashvily@incras.ru
lab head
Arseniy Lobov
contact affiliation	Yifat Merbl Lab, Dept. of Systems Immunology, Weizmann Institute of Science
contact email	arseniylobov@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD027719

PRIDE project URI

• PRIDE
  1. PXD027719
     1. Label: PRIDE project
     2. Name: LC-MS and gel-based comparison of DPSCs and PDLSCs