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PXD027712

PXD027712 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA proteomic study on the membrane protein fraction of T cells confirms high substrate selectivity for the ER translocation inhibitor cyclotriazadisulfonamide
DescriptionCyclotriazadisulfonamide (CADA) inhibits the co-translational translocation of type I integral membrane protein human CD4 (huCD4) across the ER in a signal peptide (SP)-dependent way. Previously, sortilin was identified as a secondary substrate for CADA but showed reduced CADA sensitivity as compared to huCD4. We performed a quantitative proteomic study on the membrane fraction of human T-cells to analyze how many integral membrane proteins are sensitive to CADA. We employed stable isotope labelling by amino acids in cell culture (SILAC) technique in combination with quantitative mass spectrometry on CADA-treated human T-lymphoid SUP-T1 cells expressing high levels of huCD4. In line with our previous reports, our current proteomic analysis demonstrated that only a very small subset of proteins is depleted by CADA. Our data also confirmed that cellular expression of both huCD4 and sortilin are affected by CADA-treatment of SUP-T1 cells. Furthermore, three additional targets for CADA are identified, namely, Endoplasmic Reticulum Lectin 1 (ERLEC1), Inactive Tyrosine-Protein Kinase 7 (PTK7), and DnaJ Homolog Subfamily C member 3 (DNAJC3). The sensitivity of these proteins for the translocation inhibitor CADA was confirmed by biochemical experiments including Western blots, flow cytometry and cell free in vitro translation/translocation experiments.
HostingRepositoryPRIDE
AnnounceDate2021-09-08
AnnouncementXMLSubmission_2021-09-08_06:27:49.919.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFan Liu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-08-05 05:44:28ID requested
12021-09-08 06:27:50announced
Publication List
Pauwels E, Rutz C, Provinciael B, Stroobants J, Schols D, Hartmann E, Krause E, Stephanowitz H, Sch, ü, lein R, Vermeire K, A Proteomic Study on the Membrane Protein Fraction of T Cells Confirms High Substrate Selectivity for the ER Translocation Inhibitor Cyclotriazadisulfonamide. Mol Cell Proteomics, 20():100144(2021) [pubmed]
Keyword List
submitter keyword: CD4
cyclotriazadisulfonamide (CADA)
ER co-translational translocation
signal peptide
SILAC
Contact List
Fan Liu
contact affiliationLeibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP)
contact emailfliu@fmp-berlin.de
lab head
Fan Liu
contact affiliationLeibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP)
contact emailfliu@fmp-berlin.de
dataset submitter
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