⮝ Full datasets listing

PXD027708

PXD027708 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAnalysis of RNA polymerase II interacting proteins in wild-type and spt6-1004 yeast cells.
DescriptionThe histone chaperone Spt6 is involved in promoting elongation of RNA polymerase II (RNAPII), maintaining chromatin structure, regulating co-transcriptional histone modifications, and controlling mRNA processing. These diverse functions of Spt6 are partly mediated through its interactions with RNAPII and other factors in the transcription elongation complex. In this study, we used mass spectrometry to characterize the differences in RNAPII interacting factors between wild-type cells and those depleted for Spt6, leading to the identification of proteins that depend on Spt6 for their interaction with RNAPII. In all, eight samples were processed - four genotypes (1. SPT6, RPB3-untagged; 2. SPT6, RPB3-tagged; 3. spt6-1004, RPB3-untagged; 4. spt6-1004; RPB3-tagged) in biological duplicates.
HostingRepositoryPRIDE
AnnounceDate2021-12-05
AnnouncementXMLSubmission_2021-12-05_14:55:53.789.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD027708
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRajaraman Gopalakrishnan
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-08-05 04:38:50ID requested
12021-12-05 14:55:54announced
Publication List
Gopalakrishnan R, Winston F, The histone chaperone Spt6 is required for normal recruitment of the capping enzyme Abd1 to transcribed regions. J Biol Chem, 297(4):101205(2021) [pubmed]
Keyword List
submitter keyword: S. cerevisiae, TMT, Spt6, RNAPII
Contact List
Fred Winston
contact affiliationDepartment of Genetics, Harvard Medical School, Boston MA 02115 USA
contact emailwinston@genetics.med.harvard.edu
lab head
Rajaraman Gopalakrishnan
contact affiliationHarvard Medical School
contact emailrajaraman.g@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD027708
PRIDE project URI
Repository Record List
[ + ]