PXD027704

PXD027704 is an original dataset announced via ProteomeXchange.

Title	A scalable multiplexed platform to accelerate protein interactome discovery
Description	Chromatographic fractionation-mass spectrometry (CF-MS) allows for mapping native protein-protein interactions and macromolecular complexes on a proteome-scale but is tedious and resource intensive for comparative analysis. Here, we present a more parsimonious and reproducible multiplexing CF-MS platform for measuring multi-protein assemblies across different experimental samples simultaneously with an order-of-magnitude faster than previous approaches. We used this innovative strategy to elucidate protein networks in control and breast cancer cells models in parallel within two weeks of total MS instrument time.
HostingRepository	PRIDE
AnnounceDate	2022-08-03
AnnouncementXML	Submission_2022-08-03_13:56:07.388.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pierre Havugimana
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-05 04:38:05	ID requested
⏵ 1	2022-08-03 13:56:08	announced

Havugimana PC, Goel RK, Phanse S, Youssef A, Padhorny D, Kotelnikov S, Kozakov D, Emili A, Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery. Nat Commun, 13(1):4043(2022) [pubmed]

submitter keyword: Co-Fractionation Mass Spectrometry, ion-exchange chromatography, Interactome, Multiplex, Protein-Protein Interaction, Protein Complex, Network Biology, Quantitative Proteomics, Breast Cancer, Systems Biology

Andrew Emili
contact affiliation	CNSB, Boston University, MA 02118 USA
contact email	aemili@bu.edu
lab head
Pierre Havugimana
contact affiliation	Boston University
contact email	hpierre@bu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD027704

PRIDE project URI

• PRIDE
  1. PXD027704
     1. Label: PRIDE project
     2. Name: A scalable multiplexed platform to accelerate protein interactome discovery