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PXD027700

PXD027700 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSampling and homogenization of nanoliter tissue with a nanosecond infrared laser (NIRL) for mass spectrometric proteomics
DescriptionIt was recently shown that ultrashort pulse infrared (IR) lasers, operating at the wavelength of the OH vibration stretching band of water, are highly efficient at softly sampling and homogenizing biological tissue. We utilized a nanosecond infrared laser (NIRL) for tissue sampling and homogenization with subsequent LC-MS/MS analysis for mass spectrometric proteomics. Sampling was performed with murine spleen and colon tissue with an ablation volume of 1.1 x 1.1 x0.4 mm³ (approx. 0.5 µl), determined with optical coherence tomography (OCT). The identified proteins of both tissues contained tissue type specific proteins correlating with the corresponding lists of the Human Protein Atlas. The results demonstrate that tissue sampling and homogenization of small tissue volumes less than 1 µL for subsequent mass spectrometric proteomics is feasible with a NIRL and therefore is an alternative towards sampling of tissue sections with laser capture microdissection (LCM).
HostingRepositoryPRIDE
AnnounceDate2022-02-17
AnnouncementXMLSubmission_2022-02-17_05:57:57.245.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterManuela Moritz
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-08-05 04:37:16ID requested
12022-02-17 05:57:58announced
Publication List
Hahn J, Moritz M, Vo, ß H, Pelczar P, Huber S, Schl, ü, ter H, Tissue Sampling and Homogenization in the Sub-Microliter Scale with a Nanosecond Infrared Laser (NIRL) for Mass Spectrometric Proteomics. Int J Mol Sci, 22(19):(2021) [pubmed]
Keyword List
submitter keyword: Tissue sampling
nanosecond infrared laser, laser ablation, proteomics
mass spectrometry
Contact List
Prof. Dr. Hartmut Schlüter
contact affiliationInstitute of Clinical Chemistry and Laboratory Medicine Section / Core Facility Mass Spectrometry and Proteomics
contact emailhschluet@uke.de
lab head
Manuela Moritz
contact affiliationInstitute of Clinical Chemistry and Laboratory Medicine Section / Core Facility Mass Spectrometry and Proteomics
contact emailma.moritz@uke.de
dataset submitter
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Dataset FTP location
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