PXD027700

PXD027700 is an original dataset announced via ProteomeXchange.

Title	Sampling and homogenization of nanoliter tissue with a nanosecond infrared laser (NIRL) for mass spectrometric proteomics
Description	It was recently shown that ultrashort pulse infrared (IR) lasers, operating at the wavelength of the OH vibration stretching band of water, are highly efficient at softly sampling and homogenizing biological tissue. We utilized a nanosecond infrared laser (NIRL) for tissue sampling and homogenization with subsequent LC-MS/MS analysis for mass spectrometric proteomics. Sampling was performed with murine spleen and colon tissue with an ablation volume of 1.1 x 1.1 x0.4 mm³ (approx. 0.5 µl), determined with optical coherence tomography (OCT). The identified proteins of both tissues contained tissue type specific proteins correlating with the corresponding lists of the Human Protein Atlas. The results demonstrate that tissue sampling and homogenization of small tissue volumes less than 1 µL for subsequent mass spectrometric proteomics is feasible with a NIRL and therefore is an alternative towards sampling of tissue sections with laser capture microdissection (LCM).
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_05:57:57.245.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manuela Moritz
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-05 04:37:16	ID requested
⏵ 1	2022-02-17 05:57:58	announced

Hahn J, Moritz M, Vo, ß H, Pelczar P, Huber S, Schl, ü, ter H, Tissue Sampling and Homogenization in the Sub-Microliter Scale with a Nanosecond Infrared Laser (NIRL) for Mass Spectrometric Proteomics. Int J Mol Sci, 22(19):(2021) [pubmed]

submitter keyword: Tissue sampling

nanosecond infrared laser, laser ablation, proteomics

mass spectrometry

Prof. Dr. Hartmut Schlüter
contact affiliation	Institute of Clinical Chemistry and Laboratory Medicine Section / Core Facility Mass Spectrometry and Proteomics
contact email	hschluet@uke.de
lab head
Manuela Moritz
contact affiliation	Institute of Clinical Chemistry and Laboratory Medicine Section / Core Facility Mass Spectrometry and Proteomics
contact email	ma.moritz@uke.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027700

PRIDE project URI

• PRIDE
  1. PXD027700
     1. Label: PRIDE project
     2. Name: Sampling and homogenization of nanoliter tissue with a nanosecond infrared laser (NIRL) for mass spectrometric proteomics