PXD027700 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Sampling and homogenization of nanoliter tissue with a nanosecond infrared laser (NIRL) for mass spectrometric proteomics |
Description | It was recently shown that ultrashort pulse infrared (IR) lasers, operating at the wavelength of the OH vibration stretching band of water, are highly efficient at softly sampling and homogenizing biological tissue. We utilized a nanosecond infrared laser (NIRL) for tissue sampling and homogenization with subsequent LC-MS/MS analysis for mass spectrometric proteomics. Sampling was performed with murine spleen and colon tissue with an ablation volume of 1.1 x 1.1 x0.4 mm³ (approx. 0.5 µl), determined with optical coherence tomography (OCT). The identified proteins of both tissues contained tissue type specific proteins correlating with the corresponding lists of the Human Protein Atlas. The results demonstrate that tissue sampling and homogenization of small tissue volumes less than 1 µL for subsequent mass spectrometric proteomics is feasible with a NIRL and therefore is an alternative towards sampling of tissue sections with laser capture microdissection (LCM). |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-17 |
AnnouncementXML | Submission_2022-02-17_05:57:57.245.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Manuela Moritz |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-08-05 04:37:16 | ID requested | |
⏵ 1 | 2022-02-17 05:57:58 | announced | |
Publication List
Hahn J, Moritz M, Vo, ß H, Pelczar P, Huber S, Schl, ü, ter H, Tissue Sampling and Homogenization in the Sub-Microliter Scale with a Nanosecond Infrared Laser (NIRL) for Mass Spectrometric Proteomics. Int J Mol Sci, 22(19):(2021) [pubmed] |
Keyword List
submitter keyword: Tissue sampling |
nanosecond infrared laser, laser ablation, proteomics |
mass spectrometry |
Contact List
Prof. Dr. Hartmut Schlüter |
contact affiliation | Institute of Clinical Chemistry and Laboratory Medicine Section / Core Facility Mass Spectrometry and Proteomics |
contact email | hschluet@uke.de |
lab head | |
Manuela Moritz |
contact affiliation | Institute of Clinical Chemistry and Laboratory Medicine Section / Core Facility Mass Spectrometry and Proteomics |
contact email | ma.moritz@uke.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027700
- Label: PRIDE project
- Name: Sampling and homogenization of nanoliter tissue with a nanosecond infrared laser (NIRL) for mass spectrometric proteomics