PXD027698

PXD027698 is an original dataset announced via ProteomeXchange.

Title	The oxidation site change between DVL3 wildtype and mutation
Description	First of all, we transfect FLAG-PNPO and HA-DVL3 plasmids together into 293T cells. After 48h, we harvest them with IP lysate. First day, we used special antibody to connect with high-affinity magnetic beads overnight. Second day, the beads is cleanded and then connecte with protein IP lysate, whose connection is 2 mg/mL, overnight. Last day, we use elution buffer to elution binding protein and then conduct western blotting test. Next, we use Coomassie brilliant blue to stain the SDS-page gel two hours and then clean the gels with scrubbing solution overnight in roommate temperature. We cut the lanes and send to company to analysis the bingding protein with mass specture. Based on these rusults, We find different oxidation site between DVL3 wildtype and mutation.
HostingRepository	iProX
AnnounceDate	2021-08-04
AnnouncementXML	Submission_2022-07-31_20:05:23.195.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhendong Deng
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-04 23:37:49	ID requested
⏵ 1	2022-07-31 20:05:23	announced

Dataset with its publication pending

submitter keyword: oxidation site, DVL3, wildtype, mutation

Ye Yang
contact affiliation	Nanjing University Of Chinese Medicine
contact email	yangye876@sina.com
lab head
Zhendong Deng
contact affiliation	Nanjing University Of Chinese Medicine
contact email	15951836793@163.com
dataset submitter

iProX dataset URI