PXD027650

PXD027650 is an original dataset announced via ProteomeXchange.

Title	Top-Down and Bottom-Up Proteomics Strategies for Characterization of Brazilian Pampa Snake, Bothrops pubescens, venom.
Description	The genus Bothrops is responsible for most part of envenomation accidents in Brazil. Bothrops pubescens is an endemic and neglected species in the Brazilian Pampa Biome. The characterization of its venom is essential since there is no data about it and can be helpful in the discovery of active biomolecules and for a better understanding of its action. We used top-down (TDP), native top-down, and bottom-up proteomic (BUP) approaches to characterize the venom of B. pubescens. We were able to identify 89 protein groups with the BUP approach and 40 unique proteoforms with the TDP approach, demonstrating the similarities and peculiarities of B. pubescens venom. We also identified a dimeric L-amino acid oxidase with using native TDP. Here we present for the first time a bothropic venom characterization through TDP approaches.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_06:18:39.909.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Darlene Rangel
SpeciesList	scientific name: Bothrops pubescens; NCBI TaxID: 1170827;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-29 23:47:12	ID requested
⏵ 1	2024-01-26 06:18:40	announced

Rangel DL, Melani RD, Carvalho EL, Boldo JT, Gomes Dos Santos T, Kelleher NL, Pinto PM, Venom characterization of the Brazilian Pampa snake Bothrops pubescens by top-down and bottom-up proteomics. Toxicon, 220():106937(2022) [pubmed]

10.1016/j.toxicon.2022.106937;

submitter keyword: Bothrops, snake, proteome

Darlene Lopes Rangel
contact affiliation	Universidade Federal do Pampa
contact email	darlenelrangel@gmail.com
lab head
Darlene Rangel
contact affiliation	Universidade Federal do Pampa
contact email	darlenelrangel@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD027650

PRIDE project URI

• PRIDE
  1. PXD027650
     1. Label: PRIDE project
     2. Name: Top-Down and Bottom-Up Proteomics Strategies for Characterization of Brazilian Pampa Snake, Bothrops pubescens, venom.