PXD027627

PXD027627 is an original dataset announced via ProteomeXchange.

Title	Proteomics profiling based on TMT and PRM reveal crucial biological processes in the leaves of Sophora alopecuroides under salt stress
Description	This project was based on Tandem Mass Tag (TMT) and Parallel Reaction Monitoring (PRM) technologies to study the changes in protein expression of leaves in Sophora alopecuroides under 150 mM NaCl condition treated for 3 days and 7 days. It was worthy of attention that the expression of several transporters related to the second messenger signaling pathway, such as the phosphatidylinositol signaling system, cAMP signaling pathway, and calcium signaling pathway had changed when treated for 3 d. However, the expression of some transferase, oxidoreductase, dehydrogenase was involved in the biosynthesis of flavonoids, alkaloids, phenylpropanoids and certain amino acid metabolisms had changed when treated for 7 d. This study analyzed the proteomic profiling of Sophora alopecuroides in response to salt stress using high-throughput omics technology firstly. Many potential genes were discovered that might be involved in salt stress, which laid the foundation for further research on their functions. All the results are of great significance for the researchers who are focus on the molecular genetic machnisms of abiotic stresses in plants, especially under salt stress.
HostingRepository	PRIDE
AnnounceDate	2022-02-14
AnnouncementXML	Submission_2022-02-14_02:21:18.820.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tian-li Ma
SpeciesList	scientific name: Sophora alopecuroides; NCBI TaxID: 200492;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-28 04:38:52	ID requested
⏵ 1	2022-02-14 02:21:19	announced

Ma TL, Li WJ, Hong YS, Zhou YM, Tian L, Zhang XG, Liu FL, Liu P, TMT based proteomic profiling of Sophora alopecuroides leaves reveal flavonoid biosynthesis processes in response to salt stress. J Proteomics, 253():104457(2022) [pubmed]

submitter keyword: Proteomics, TMT, PRM, Sophora alopecuroides, Salt stress

Ping Liu
contact affiliation	Key Laboratory of Modern Molecular Breeding of Advantageous Crops of Ningxia, School of Agriculture, Ningxia University, Yinchuan, Ningxia 750021, China
contact email	liupnxdx@126.com
lab head
Tian-li Ma
contact affiliation	Key Laboratory of Modern Molecular Breeding of Advantageous Crops of Ningxia, School of Agriculture, Ningxia University, Yinchuan, Ningxia 750021, China
contact email	tianlima2018@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027627

PRIDE project URI

• PRIDE
  1. PXD027627
     1. Label: PRIDE project
     2. Name: Proteomics profiling based on TMT and PRM reveal crucial biological processes in the leaves of Sophora alopecuroides under salt stress