PXD027623

PXD027623 is an original dataset announced via ProteomeXchange.

Title	Multistep mechanism of G-quadruplex resolution during DNA replication
Description	G-quadruplex (or G4) structures are non-canonical DNA structures that form in guanine-rich sequences and threaten genome stability when not properly resolved. G4 unwinding occurs during S phase via an unknown mechanism. Using Xenopus egg extracts, we define a three-step G4 unwinding mechanism that acts during DNA replication. First, the replicative helicase (CMG) stalls at a leading strand G4 structure. Second, the DHX36 helicase mediates the bypass of the CMG past the intact G4 structure, which allows approach of the leading strand to the G4. Third, G4 structure unwinding by the FANCJ helicase enables the DNA polymerase to synthesize past the G4 motif. A G4 on the lagging strand template does not stall CMG, but still requires active DNA replication for unwinding. DHX36 and FANCJ have partially redundant roles, conferring robustness to this pathway. Our data reveal a novel genome maintenance pathway that promotes faithful G4 replication thereby avoiding genome instability.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_05:56:33.423.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Harm Post
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-28 02:31:26	ID requested
⏵ 1	2022-02-17 05:56:34	announced

Sato K, Martin-Pintado N, Post H, Altelaar M, Knipscheer P, Multistep mechanism of G-quadruplex resolution during DNA replication. Sci Adv, 7(39):eabf8653(2021) [pubmed]

submitter keyword: G-quadruplex,G4, G4 resolution, DNA replication, FANCJ, DHX36, helicases

Puck Knipscheer
contact affiliation	Oncode Institute, Hubrecht Institute-KNAW & University Medical Center Utrecht, Uppsalalaan 8, Utrecht, 3584 CT, The Netherlands
contact email	p.knipscheer@hubrecht.eu
lab head
Harm Post
contact affiliation	Biomolecular Mass Spectrometry and Proteomics Group
contact email	h.post@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027623

PRIDE project URI

• PRIDE
  1. PXD027623
     1. Label: PRIDE project
     2. Name: Multistep mechanism of G-quadruplex resolution during DNA replication