PXD027617

PXD027617 is an original dataset announced via ProteomeXchange.

Title	Targeted Protein Acetylation in Cells Using Heterobifunctional Molecules
Description	Protein acetylation is a central event in orchestrating diverse cellular processes. However, current strategies to investigate protein acetylation in cells are often non-specific or lack temporal and magnitude control. Here, we developed an acetylation tagging system, AceTAG, to induce acetylation of targeted proteins. The AceTAG system utilizes bifunctional molecules to direct the lysine acetyltransferase p300/CBP to proteins fused with the small protein tag FKBP12F36V, resulting in their induced acetylation. Using AceTAG, we induced targeted acetylation of a diverse array of proteins in cells, specifically histone H3.3, the NF-B subunit p65/RelA, and the tumor suppressor p53. We demonstrate that targeted acetylation with the AceTAG system is rapid, selective, reversible, and can be controlled in a dose-dependent fashion. AceTAG represents a useful strategy to modulate protein acetylation and will enable the exploration of targeted acetylation in basic biological and therapeutic contexts.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_07:05:52.707.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Li Yun Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-28 02:30:27	ID requested
⏵ 1	2024-01-26 07:05:53	announced

Wang WW, Chen LY, Wozniak JM, Jadhav AM, Anderson H, Malone TE, Parker CG, Targeted Protein Acetylation in Cells Using Heterobifunctional Molecules. J Am Chem Soc, 143(40):16700-16708(2021) [pubmed]

10.1021/jacs.1c07850;

submitter keyword: targeted acetylation, acetylproteomics, p300/CBP, heterobifunctional molecules

Christopher G. Parker
contact affiliation	Assistant Professor, Department of Chemistry, The ScrippsResearch Institute
contact email	cparker@scripps.edu
lab head
Li Yun Chen
contact affiliation	The Scripps Research Institute
contact email	lichen@scripps.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD027617

PRIDE project URI

• PRIDE
  1. PXD027617
     1. Label: PRIDE project
     2. Name: Targeted Protein Acetylation in Cells Using Heterobifunctional Molecules