PXD027611

PXD027611 is an original dataset announced via ProteomeXchange.

Title	Identifying and characterising Thrap3, Bclaf1 and Erh direct interactions using cross-linking mass spectrometry
Description	Cross-linking mass spectrometry (XL-MS) is a powerful technology capable of yielding structural insights across the complex cellular protein interaction network. We performed XL-MS using MS-cleavable cross-linker disuccinimidyl sulfoxide (DSSO) on endogenous affinity-purified BAF complex. We identified numerous crosslinks between three BAF co-purifying proteins, namely Thrap3, Bclaf1 and Erh. Our data suggests that Bclaf1, Erh and Thrap3 interact closely and might represent a stable complex. The XL data allowed us to map interaction surfaces on Thrap3 and B, which overlap with the non-disordered portions of both proteins.
HostingRepository	PRIDE
AnnounceDate	2021-09-24
AnnouncementXML	Submission_2021-09-24_06:24:23.528.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Wright
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-27 22:37:21	ID requested
⏵ 1	2021-09-24 06:24:23	announced

Dataset with its publication pending

submitter keyword: endogenous complexes, XL-MS, CLMS cross-linking mass spectrometry, DSSO, Thrap3, Bclaf1, Erh

Jyoti Choudhary
contact affiliation	The Institute of Cancer Research
contact email	jyoti.choudhary@icr.ac.uk
lab head
James Wright
contact affiliation	The Institute of Cancer Research
contact email	james.wright@icr.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD027611

PRIDE project URI

• PRIDE
  1. PXD027611
     1. Label: PRIDE project
     2. Name: Identifying and characterising Thrap3, Bclaf1 and Erh direct interactions using cross-linking mass spectrometry