PXD027608

PXD027608 is an original dataset announced via ProteomeXchange.

Title	The Effect of Temperature Conditioning (9°C and 20°C) on the Proteome of Steinernema carpocapsae and Heterorhabditis megidis Infective Juveniles
Description	Entomopathogenic nematodes (Rhabditida; Steinernematidae and Heterorhabditidae) are insect parasites which are of economic importance due to their use as biocontrol agents. The third larval stage, infective juveniles (IJs) leave the natal host and move in the soil to locate a new insect host. Both families are associated with mutualistic bacteria, which are released into the haemolymph of the host insect, killing the insect and providing nutrition for nematode development and reproduction, and are thus categorised as entomopathogenic nematodes. Similarities between these two families are due to convergent evolution associated with this lifestyle, rather than common ancestry. Heterorhabditids are closely related to the vertebrate parasites Strongylida (Adams and Nguyen, 2002) and to Caenorhabditis elegans, whereas steinernematids are more closely related to Strongyloididae. The IJ stage of parasitic nematodes is analogous to the dauer stage of C. elegans; both are developmentally arrested, stress resistant stages that disperse to colonise new hosts or food resources, respectively. By investigating the molecular mechanisms and consequences of temperature acclimation and aging in EPN IJs, insights into survival and the changes induced by low temperature exposure may be gained. Label free quantitative (LFQ) massspectrometery-based proteomics facilitates the identification and quantification of thousands of proteins in a single run. Such data allows for the comparison of the proteomes of EPN IJs after conditioning via gene ontology mapping and functional enrichment analysis. Understanding how the IJ proteome is affected by temperature and aging can provide a molecular basis for the wide array of phenotypes and behaviours these organisms may adopt. This study aims to provide proteomic data which may elucidate the molecular mechanisms underlying the phenotypic plasticity which EPN IJs of two distantly related species exhibit.
HostingRepository	PRIDE
AnnounceDate	2022-03-27
AnnouncementXML	Submission_2022-03-27_05:40:10.798.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Carolan
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Steinernema carpocapsae; NCBI TaxID: 34508; scientific name: Heterorhabditis megidis; NCBI TaxID: 52065;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-27 22:35:33	ID requested
⏵ 1	2022-03-27 05:40:11	announced

Dataset with its publication pending

submitter keyword: Steinernema carpocapsae, Heterorhabditis megidis, temperature conditioning, enomopathogenic nematodes

James Carolan
contact affiliation	Maynooth University, Ireland
contact email	james.carolan@mu.ie
lab head
James Carolan
contact affiliation	Biology
contact email	james.carolan@nuim.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027608
     1. Label: PRIDE project
     2. Name: The Effect of Temperature Conditioning (9°C and 20°C) on the Proteome of Steinernema carpocapsae and Heterorhabditis megidis Infective Juveniles