PXD027582

PXD027582 is an original dataset announced via ProteomeXchange.

Title	Absence of COVID-19-associated Changes in Coagulation and Thrombosis in the Ferret Model
Description	Many patients who are diagnosed with coronavirus disease 2019 (COVID-19) suffer from venous thromboembolic complications despite the use of stringent anticoagulant prophylaxis. Studies on the exact mechanism(s) underlying thrombosis in COVID-19 are limited as animal models commonly used to study venous thrombosis pathophysiology (i.e. rats and mice) are naturally not susceptible to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Ferrets are susceptible to SARS-CoV-2 infection, successfully used to study virus transmission, and were previously used to study activation of coagulation and thrombosis during influenza virus infection. Here, we used plasma and lung material from SARS-CoV-2-inoculated ferrets to explore their use in studying COVID-19-associated changes in coagulation and thrombosis. Lungs of ferrets inoculated intranasally with SARS-CoV-2 demonstrated alveolar septa that were mildly expanded by macrophages, and diffuse interstitial histiocytic pneumonia. However, no macroscopical or microscopical evidence of vascular thrombosis in the lungs of SARS-CoV-2-inoculated ferrets was found. Longitudinal plasma profiling using a mass spectrometry-based approach revealed minor differences in plasma protein profiles in SARS-CoV-2-inoculated ferrets up to 2 weeks post-infection. Apart from fibrinogen, the majority of plasma coagulation factors were stable and demonstrated a low coefficient of variation. We conclude that while ferrets are an essential and well-suited animal model to study SARS-CoV-2 transmission, their use to study SARS-CoV-2-related changes relevant to thrombotic disease is limited.
HostingRepository	PRIDE
AnnounceDate	2022-07-19
AnnouncementXML	Submission_2022-07-19_06:12:45.111.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	iris Kreft
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Mustela putorius furo; NCBI TaxID: 9669;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-27 07:04:08	ID requested
⏵ 1	2022-07-19 06:12:45	announced

Kreft IC, Winiarczyk RRA, Tanis FJ, van der Zwaan C, Schmitz KS, Hoogendijk AJ, de Swart RL, Moscona A, Porotto M, Salvatori DCF, de Vries RD, de Maat MPM, van den Biggelaar M, van Vlijmen BJM, Absence of COVID-19-associated changes in plasma coagulation proteins and pulmonary thrombosis in the ferret model. Thromb Res, 210():6-11(2022) [pubmed]

ProteomeXchange project tag: Covid-19

submitter keyword: COVID-19, Ferrets, Mass spectrometry, Proteomics, Thrombosis

Maartje van den Biggelaar
contact affiliation	Lab head
contact email	m.vandenbiggelaar@sanquin.nl
lab head
iris Kreft
contact affiliation	Sanquin Reserach
contact email	i.kreft@sanquin.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027582
     1. Label: PRIDE project
     2. Name: Absence of COVID-19-associated Changes in Coagulation and Thrombosis in the Ferret Model