PXD027578

PXD027578 is an original dataset announced via ProteomeXchange.

Title	Quantitative Chemoproteomic Profiling with Data-Independent Acquisition-based Mass Spectrometry
Description	Activity-based protein profiling (ABPP) has emerged as a powerful and versatile tool to enable annotation of protein functions and discovery of targets of bioactive ligands in complex biological systems. It utilizes chemical probes to covalently label functional sites in proteins so that they can be enriched for mass spectrometry (MS)-based quantitative proteomics analysis. However, the semi-stochastic nature of data-dependent acquisition (DDA) and high cost associated with isotopically encoded quantification reagents compromise the power of ABPP in multidimensional analysis and high-throughput screening, when a large number of samples need to be quantified in parallel. Here, we combine the data-independent acquisition (DIA) mass spectrometry with ABPP to develop an efficient label-free quantitative chemical proteomic method, DIA-ABPP, with good reproducibility and high accuracy for multiplex quantification. We demonstrated the power of DIA-ABPP for comprehensive profiling of functional cysteineome in three distinct applications, including dose-dependent quantification of cysteines’ sensitivity toward a reactive metabolite, screening of ligandable cysteines with a covalent fragment library, and profiling of cysteinome fluctuation in circadian clock cycles. DIA-ABPP will open new opportunities for in-depth and multidimensional profiling of functional proteomes and interactions with bioactive small molecules in complex biological systems.
HostingRepository	iProX
AnnounceDate	2021-07-27
AnnouncementXML	Submission_2022-04-07_22:13:07.590.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fan Yang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-27 00:48:03	ID requested
⏵ 1	2022-04-07 22:13:08	announced

Yang F, Jia G, Guo J, Liu Y, Wang C, Quantitative Chemoproteomic Profiling with Data-Independent Acquisition-Based Mass Spectrometry. J Am Chem Soc, 144(2):901-911(2022) [pubmed]

submitter keyword: Activity-based protein profiling, Data-Independent acquisition

Chu Wang
contact affiliation	Peking University
contact email	chuwang@pku.edu.cn
lab head
Fan Yang
contact affiliation	Peking University
contact email	fan_yang@pku.edu.cn
dataset submitter

iProX dataset URI