PXD027572

PXD027572 is an original dataset announced via ProteomeXchange.

Title	A novel approach for the discovery of biomarkers of radiotherapy response in breast cancer
Description	Radiotherapy is a commonly used treatment modality for the local control of breast cancer. However, the clinical signs of radiotherapy response are often not apparent for several weeks post-treatment. We currently lack tools to predict and/or monitor tumor responses during treatment. The aim of this study was to identify tumor secreted biomarkers of radiotherapy response. Estrogen receptor positive (ER+) MCF-7 cells were serum-starved for 2 h, and were then exposed to various doses of radiation (0, 2, 4, 6, 8 or 10 Gy). Conditioned media (CM) was harvested at 1, 2, 4, 8 and 24 h post-radiation for each radiation dose. Samples underwent processing for liquid chromatography-mass spectrometry (LC-MS) following collection. 33 proteins at the 24 h time point were found to have significantly increased secretion levels (up to 12-fold) at all radiation doses compared to untreated cells. To validate the secretomic results and to further investigate the potential use of these proteins as biomarkers of radiosensitivity, the secreted protein levels of candidate biomarkers were assessed through western blot (WB). WB analysis was performed using CM samples to assess the secretion levels from parental and radioresistant (RR) cell lines (developed within our lab) 24 h after the cells had received a single radiation dose of 2 Gy. Secretion levels of our candidate biomarkers were found to be significantly increased from the radiosensitive MCF-7 cells treated with 2 Gy of radiation at 24 h compared to 24 h untreated controls. In comparison, candidate biomarker levels in the CM samples from untreated and radiation-treated MCF-7 RR cells remained low. Currently there are no validated predictive biomarkers to monitor radiotherapy responses during treatment. These biomarkers could have a clinical role in personalising radiotherapy dosing schedules and durations for solid tumours in the neoadjuvant and palliative setting.
HostingRepository	PRIDE
AnnounceDate	2021-08-13
AnnouncementXML	Submission_2021-08-13_01:31:12.583.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jimi Wills
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-26 06:24:40	ID requested
⏵ 1	2021-08-13 01:31:13	announced

Meehan J, Gray M, Mart, í, nez-P, é, rez C, Kay C, McLaren D, Turnbull AK, Tissue- and Liquid-Based Biomarkers in Prostate Cancer Precision Medicine. J Pers Med, 11(7):(2021) [pubmed]

submitter keyword: Human breast cancer, Radiotherapy, Radiosensitivity biomarkers, Secretome, Radioresistance

Arran Turnbull
contact affiliation	Translational Oncology Research Group, Institute of Genetics and Cancer, Western General Hospital, University of Edinburgh, Scotland, United Kingdom
contact email	a.turnbull@ed.ac.uk
lab head
Jimi Wills
contact affiliation	University of Edinburgh
contact email	jimi.wills@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD027572

PRIDE project URI

• PRIDE
  1. PXD027572
     1. Label: PRIDE project
     2. Name: A novel approach for the discovery of biomarkers of radiotherapy response in breast cancer