PXD027571

PXD027571 is an original dataset announced via ProteomeXchange.

Title	The analysis of the α2,6-sialylated proteins in endometrium from porcine gestational day 15 by LC-MS/MS
Description	Implantation is the attachment of embryo in the endometrium. Failure in implantation is a major cause of early pregnancy loss. During implantation, the temporal uterine lumen closure can help embryo attach to the uterus. In pigs, extending of endometrial folds to form interlocking finger-like projections is a main cause leads to uterine lumen closure during attachment time, but the underlying mechanisms are largely unknown. Our data reveal that pig uterine luminal epithelium (LE) migrate in coordinated groups during extending of endometrial folds. Moreover, the MALDI-TOF MS based N-glycomic characterization of porcine endometrium revealed α2,6-linked sialic acid are highly expressed in pig uterine LE during extending of endometrial folds. To investigated the mechanisms by which α2,6-sialylated proteins in formation of the endometrial folding during implantation in pigs, the α2,6-sialylated proteins in pig uterine LE were characterized by proteomic analysis and those proteins that are involved in cell adhesion, such as E-cadherin, were detected. Finally, our in vivo and in vitro data show that α2,6-sialylation of E-cadherin occurs in accompany with collective epithelial migration. The results provide new insight into the mechanism of pig implantation by identifying that α2,6-sialylation of cell adhesion molecules may participate in formation of extending of endometrial folds through promoting of collective migration of uterine LE.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_05:41:39.349.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kun Han
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-26 06:20:40	ID requested
⏵ 1	2022-02-17 05:41:40	announced

Han K, Wang F, Yue Y, Tan X, Tian M, Miao Y, Zhao S, Dong W, Yu M, Glycomics reveal that ST6GAL1-mediated sialylation regulates uterine lumen closure during implantation. Cell Prolif, 55(1):e13169(2022) [pubmed]

submitter keyword: endometrium, porcine pregnancy, α2,6-sialylated proteins, LC-MS/MS

Mei Yu
contact affiliation	Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
contact email	yumei@mail.hzau.edu.cn
lab head
Kun Han
contact affiliation	Huazhong Agricultural University
contact email	hankun@webmail.hzau.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027571

PRIDE project URI

• PRIDE
  1. PXD027571
     1. Label: PRIDE project
     2. Name: The analysis of the α2,6-sialylated proteins in endometrium from porcine gestational day 15 by LC-MS/MS