PXD027566

PXD027566 is an original dataset announced via ProteomeXchange.

Title	Plasmodium falciparum exported protein, PTP7, co-immunoprecipitation LC-MSMS
Description	The malaria parasite, Plasmodium falciparum, traffics the virulence protein erythrocyte membrane protein 1 (EMP1) to the surface of infected red blood cells (RBCs) via membranous organelles known as the Maurer’s clefts. How EMP1 is trafficked from the clefts to its site of action at the RBC surface is poorly understood. Here we build upon previous studies (DOI: 10.1128/mBio.03320-19) which had identified the exported Plasmodium protein PF3D7_0301700, or PTP7, as a protein of interest in post-cleft EMP1 trafficking. Transfectants expressing PTP7-GFP confirmed PTP7’s localization to the cleft and association with vesicles and soluble chaperoned complexes also implicated in EMP1 trafficking. Co-immunoprecipitation and mass spectrometry of PTP7-GFP confirmed associations with Maurer’s cleft, vesicle, and chaperone complex proteins as observed by light and electron microscopy. This experiment also expands the network map of exported protein-protein associations characterized to date.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:52:30.861.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olivia Carmo
SpeciesList	scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: 36329;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-26 02:04:42	ID requested
1	2022-07-04 04:16:00	announced
⏵ 2	2023-11-14 08:52:31	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: exported protein, LC-MSMS,Plasmodium falciparum

Matthew W. A. Dixon
contact affiliation	University of Melbourne
contact email	matthew.dixon@unimelb.edu.au
lab head
Olivia Carmo
contact affiliation	University of Melbourne
contact email	ocarmo@student.unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD027566

PRIDE project URI

• PRIDE
  1. PXD027566
     1. Label: PRIDE project
     2. Name: Plasmodium falciparum exported protein, PTP7, co-immunoprecipitation LC-MSMS