PXD027552

PXD027552 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry analysis of the blood plasma and serum samples from healthy donors
Description	Previously, we published a dataset of human blood plasma and serum samples of 10 healthy males and 10 healthy females, fractionated on a set of sorbents (cation exchange Toyopearl CM-650M, CM Bio-Gel A, SP Sephadex C-25 and anion exchange QAE Sephadex A-25) and analyzed by LC-MS/MS individually and pooled in equal amounts (Supplementary Table S1, Sheet 1) [33]. We focused on those peptides that could be found in the blood plasma and serum using a standard search against the database of human proteins (UniProt Knowledgebase, taxon human). The combination of search engines MASCOT and X! Tandem, based on Scaffold 4 software with FDR less than 1%, allowed us to identify 15,530 unique peptides that belong to 2,127 protein groups (Table 1, Supplementary Table S1, Sheets 2 and 3). The Venn diagram shows that slightly less than 40% of the peptides are found in all four analyzed samples (Figures 1A and 1B). About 70% of the peptides are found in at least two samples. The accumulation diagram (Figure 1C) shows that the number of new unique peptides does not reach a plateau after repeated analyses of the same plasma sample — even after six runs. The high variability of the plasma and serum peptidome most likely indicates its sophisticated composition. 33. Arapidi, G. et al. Peptidomics dataset: Blood plasma and serum samples of healthy donors fractionated on a set of chromatography sorbents. Data Brief 18, 1204–1211 (2018).
HostingRepository	PRIDE
AnnounceDate	2024-06-14
AnnouncementXML	Submission_2024-06-14_08:50:32.548.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027552
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Georgij Arapidi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-25 15:39:08	ID requested
⏵ 1	2024-06-14 08:50:32	announced

10.6019/PXD027552;

submitter keyword: peptidomics, mass spectrometry, blood plasma and serum,Healthy donors

Georgij Pavlovich Arapidi
contact affiliation	Systems Biology Laboratory, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
contact email	arapidi@gmail.com
lab head
Georgij Arapidi
contact affiliation	Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
contact email	arapidi@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027552
     1. Label: PRIDE project
     2. Name: Mass spectrometry analysis of the blood plasma and serum samples from healthy donors