PXD027551

PXD027551 is an original dataset announced via ProteomeXchange.

Title	Integrated metabolomics and proteomics dynamics of serum samples reveals dietary Zeolite clinoptilolite supplementation restores energy balance in high yielding dairy cows
Description	Dairy cows can suffer from a negative energy balance (NEB) during their transition from dry period to early lactation when feed intake does not sufficiently meet the energy requirements for body maintenance and homeostasis. The subsequent metabolic changes can increase the risk of postpartum diseases such as clinical ketosis, mastitis and fatty liver. Zeolite clinoptilolite (CPL), due to its ion-exchange property, has been often used to treat NEB and other disorders such as rumen digestion in animals in farming. However, limited information is available on the dynamics of global metabolomics and proteomic profiles in serum that could give us a better understanding on the associated altered biological pathways in response to CPL. Thus, in the present study, a total 64 cows randomly assigned to two groups: control (n=32) and CPL-treated (n=32) at time points of 30 days (n=8) and 10 days (n=8) prepartum stage and 5 days (n=8) and 26 (n=8) days postpartum stage. Labelled proteomics and untargeted metabolomics were performed following the determination of β-hydroxy-butyric acid (BHB) concentration in the samples indicating NEB. A total of 64 proteins were differentially expressed with a significant cohort appearing to play key roles in restoring the EB after CPL supplementation. In addition, 21 differentially expressed metabolites were chosen of 83 identified metabolites based on their high correlation with BHB. Joint pathway and interaction analysis revealed cross-talks among potential candidates such as valproic acid, leucic acid, glycerol, fibronectin and kinninogen-1 which could be responsible for restoring associated complications of NEB such as infertility or infection like mastitis. By using global proteomics and metabolomics strategy, the present study concluded that the CPL supplementation could lower NEB and restore energy balance in just a few weeks and explained the possible underlying pathways employed by the CPL.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_05:39:43.058.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sudipa Maity
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-25 15:38:39	ID requested
⏵ 1	2022-02-17 05:39:43	announced

Maity S, Rubi, ć I, Kule, š J, Horvati, ć A, Đ, uri, č, i, ć D, Samard, ž, ija M, Ljubi, ć BB, Turk R, Gra, č, ner D, Ma, ć, e, š, i, ć N, Valpoti, ć H, Mrljak V, Integrated Metabolomics and Proteomics Dynamics of Serum Samples Reveals Dietary Zeolite Clinoptilolite Supplementation Restores Energy Balance in High Yielding Dairy Cows. Metabolites, 11(12):(2021) [pubmed]

submitter keyword: Negative energy balance, zeolite clinoptilolite, proteomics, metabolomics, cows, serum

Vladimir Mrljak
contact affiliation	Faculty of Veterinary Medicine, University of Zagreb, Croatia
contact email	vmrljak@vef.hr
lab head
Sudipa Maity
contact affiliation	Tulane University
contact email	sudipabiotech.iitr@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027551
     1. Label: PRIDE project
     2. Name: Integrated metabolomics and proteomics dynamics of serum samples reveals dietary Zeolite clinoptilolite supplementation restores energy balance in high yielding dairy cows