PXD027546

PXD027546 is an original dataset announced via ProteomeXchange.

Title	The heat-stable proteome contains a tightly associated LEA protein network during seed germination in Arabidopsis
Description	Seed germination involves transition from desiccation tolerance to sensitivity during early stages of seedling establishment accompanied by comprehensive changes of metabolome and proteome. Germination of Arabidopsis seeds was investigated over 72 h with special focus on the heat-stable proteome including late embryogenesis abundant (LEA) proteins together with changes of primary metabolites. Six metabolites in dry seeds important for seed longevity decreased during germination and seedling establishment, while all other metabolites increased simultaneously with activation of growth and development. In the heat stable proteome an almost equal fractioning of ordered and intrinsically disordered proteins (IDP) was discovered. Highly disordered proteins were assigned to functional bins development, protein, RNA and stress. Thermal stable proteins were related to a multitude of active biological processes. The majority of LEA proteins decreased during germination and seedling establishment in parallel to reduced desiccation tolerance. Five germination specific dehydrins were identified most likely providing stress tolerance. Network analysis of common proteins and metabolites over all time points revealed a tight network of LEA proteins with five hub LEAs with high connectivity. Connections to proteins such as Rubisco large subunit and seed storage proteins were discovered. Insights into the heat-stable proteome – metabolome network during seed germination are provided.
HostingRepository	PRIDE
AnnounceDate	2021-07-29
AnnouncementXML	Submission_2021-07-29_02:01:01.956.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michał Górka
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-25 15:35:13	ID requested
⏵ 1	2021-07-29 02:01:02	announced

Dataset with its publication pending

submitter keyword: late embryogenesis abundant, Arabidopsis, seed germination, metabolomics, heat-stable proteome, dehydrins, gene expression

Ellen Zuther
contact affiliation	MPI für Molekulare Pflanzenphysiologie, Potsdam, Head of Zentrale Infrastrukturgruppe Genomics und Transkript Profiling
contact email	zuther@mpimp-golm.mpg.de
lab head
Michał Górka
contact affiliation	Celon Pharma
contact email	michal.gorka.ofc@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD027546

PRIDE project URI

• PRIDE
  1. PXD027546
     1. Label: PRIDE project
     2. Name: The heat-stable proteome contains a tightly associated LEA protein network during seed germination in Arabidopsis