PXD027541

PXD027541 is an original dataset announced via ProteomeXchange.

Title	Pig,extracellular vesicles,LC-MS
Description	Seminal plasma contains a large number of extracellular vesicles (EVs). However, the roles of these EVs and their interactions with sperm are not clear. To identify the important molecules affecting sperm motility in EVs, we performed proteomics analysis of SPEVs from the two groups with different sperm motilities. In total, 1435 exosomal proteins covered a broad range of protein classes, such as enzymes, cytoskeletal proteins, extracellular proteins and transcription factors. We identified 76 DEPs (differentially expressed protein) from the SPEVs of boars with high- and low-sperm motility phenotypes (|FC| ≥1.5, P≤ 0.05). A total of 38 proteins were downregulated in the L group compared with the H group, while 6 proteins were significantly upregulated. In addition, 28 proteins were identified only in the H group, and 4 proteins were identified only in the L group. The GO and KEGG analyses showed that the DEPs were associated primarily with negative regulation of cell motility, extracellular exosomes, the extracellular space, cell-cell adhesion and phagosome biological activities. The present fndings represent a major and novel contribution to the study of boar seminal extracellular vesicles proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:30:16.294.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yu Zhang
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	No PTMs are included in the dataset
Instrument	Thermo Fisher Scientific instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-25 14:46:21	ID requested
1	2021-11-04 09:27:56	announced
2	2024-02-12 09:03:50	announced	2024-02-12: Updated project metadata.
⏵ 3	2024-10-22 05:30:17	announced	2024-10-22: Updated project metadata.

Ding Y, Ding N, Zhang Y, Xie S, Huang M, Ding X, Dong W, Zhang Q, Jiang L, . Front Cell Dev Biol, 9():736864(2021) [pubmed]

10.3389/fcell.2021.736864;

submitter keyword: seminal plasma,LC-MS,extracellular vesicles,Pig

Jiang li
contact affiliation	National Engineering Laboratory For Animal Breeding,China Agricultural University,No 2Yuanmingyuan West Rd,Beijing 10093,China
contact email	lijiang@cau.edu.cn
lab head
Yu Zhang
contact affiliation	13120457265
contact email	203127671@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD027541

PRIDE project URI

• PRIDE
  1. PXD027541
     1. Label: PRIDE project
     2. Name: Pig,extracellular vesicles,LC-MS