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PXD027530

PXD027530 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAdsorptive microtiter plates as solid supports in affinity purification workflows
DescriptionAffinity ligands such as antibodies are widely used in (bio)medical research for purifying proteins from complex biological samples. These ligands are generally immobilized onto solid supports which facilitate the separation of captured protein from sample matrix. Adsorptive microtiter plates are commonly used as solid supports prior to immunochemical detection (e.g. immunoassays) but hardly ever prior to LC-MS-based detection, and here we describe some applications, opportunities, and challenges of corresponding workflows.
HostingRepositoryPRIDE
AnnounceDate2021-11-25
AnnouncementXMLSubmission_2021-11-25_04:48:07.008.xml
DigitalObjectIdentifierhttp://dx.doi.org/10.6019/PXD027530
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterHorvatovich Péter
SpeciesList scientific name: Murine leukemia virus isolates; NCBI TaxID: 368798;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-25 14:31:05ID requested
12021-11-25 04:48:07announced
Publication List
Klont F, Kwiatkowski M, Faiz A, van den Bosch T, Pouwels SD, Dekker FJ, Ten Hacken NHT, Horvatovich P, Bischoff R, Adsorptive Microtiter Plates As Solid Supports in Affinity Purification Workflows. J Proteome Res, 20(11):5218-5221(2021) [pubmed]
Keyword List
submitter keyword: immuno capture, LC-MS/MS, Transcription factor p65, Peptidyl-prolyl cis-trans isomerase FKBP5
Contact List
Peter Horvatovich
contact affiliationUniversity of Groningen
contact emailp.l.horvatovich@rug.nl
lab head
Horvatovich Péter
contact affiliationFaculty of Science and Engineering, Groningen Research Institute of Pharmacy, Analytical Biochemistry, University of Groningen, 9713 AV Groningen, The Netherlands
contact emailp.l.horvatovich@rug.nl
dataset submitter
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PRIDE project URI
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