PXD027529

PXD027529 is an original dataset announced via ProteomeXchange.

Title	Kinase-catalyzed Biotinylation to Map Cell Signaling Pathways (K-BMAPS): Application to Epidermal Growth Factor Signaling
Description	Cell signaling involves a network of protein-protein interactions and post-translational modifications that govern cellular responses to environmental cues. To understand and ultimately modulate these signaling pathways to confront disease, the complex web of proteins that becomes phosphorylated after extracellular stimulation has been studied using mass spectrometry-based proteomics methods. To complement prior work and fully characterize all phosphorylated proteins after stimulation of cell signaling, we developed K-BMAPS (Kinase-catalyzed Biotinylation to MAP Signaling), which utilizes ATP-biotin as a kinase cosubstrate to biotin label substrates. As a first application of K-BMAPS, the well-characterized epidermal growth factor receptor (EGFR) kinase signaling pathway was monitored by treating EGF-stimulated HeLa lysates with ATP-biotin, following by streptavidin enrichment and quantitative mass spectrometry analysis. Based on the dynamic phosphoproteins identified, a pathway map was developed considering functional categories and known interactors of EGFR. Remarkably, 94% of K-BMAPS hit proteins were included in the EGFR pathway map. With many proteins involved in transcription, translation, cell adhesion, and GTPase signaling, K-BMAPS identified phosphoproteins associated with late and continuous signaling events. In summary, K-BMAPS is a powerful tool to map the dynamic phosphorylation governing cell signaling pathways.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_03:17:06.927.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027529
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jordan Burton
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-25 14:03:19	ID requested
⏵ 1	2021-11-03 03:17:07	announced

Ramanayake-Mudiyanselage V, Embogama DM, Pflum MKH, Kinase-Catalyzed Biotinylation to Map Cell Signaling Pathways: Application to Epidermal Growth Factor Signaling. J Proteome Res, 20(10):4852-4861(2021) [pubmed]

submitter keyword: cell signaling

kinase

ATP analog

ATP-biotin

proteomics

interactome

Mary Kay Pflum
contact affiliation	Department of Chemistry, Wayne State University, United States
contact email	pflum@wayne.edu
lab head
Jordan Burton
contact affiliation	Department of Chemistry Institute of Environmental Health Sciences Wayne State University
contact email	jordan.burton@wayne.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027529
     1. Label: PRIDE project
     2. Name: Kinase-catalyzed Biotinylation to Map Cell Signaling Pathways (K-BMAPS): Application to Epidermal Growth Factor Signaling