PXD027524

PXD027524 is an original dataset announced via ProteomeXchange.

Title	Ubiquitination in treated T. cruzi parasites
Description	T. cruzi epimastigotes in the logarithmic growth phase (3×106 cells mL-1) were incubated for 12h with bortezomib (1.8 µM), GNF6702 (2.9 µM) or compound 1 (24 µM), equivalent to 8× the EC50 values of each compound. Controls were incubated in the presence of diluent (DMSO). Cells were harvested by centrifugation (1912g, 15 min, 4 °C) and washed with ice-cold PBS (1912g, 5 min, 4 °C), and finally, the cell pellets were resuspended in 1.5 mL of ice-cold lysis buffer (1 mM EDTA, 1 mM DTT, 100 μM TLCK, and 1× Roche EDTA-free cOmplete protease inhibitor cocktail in 50 mM potassium phosphate buffer, pH 7.4). Cell suspensions were submitted to 3 freeze–thaw cycles in a dry ice/ethanol bath to biologically inactivate the parasites and then lysed using the One ShotTM Cell disruptor (Constant Systems, UK) at 30 kpsi.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_05:35:59.882.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Victoriano Corpas-Lopez
SpeciesList	scientific name: Trypanosoma cruzi Dm28c; NCBI TaxID: 1416333;
ModificationList	ubiquitinylated lysine
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-25 13:53:08	ID requested
⏵ 1	2022-02-17 05:36:00	announced

Lima ML, Tulloch LB, Corpas-Lopez V, Carvalho S, Wall RJ, Milne R, Rico E, Patterson S, Gilbert IH, Moniz S, MacLean L, Torrie LS, Morgillo C, Horn D, Zuccotto F, Wyllie S, Identification of a Proteasome-Targeting Arylsulfonamide with Potential for the Treatment of Chagas' Disease. Antimicrob Agents Chemother, 66(1):e0153521(2022) [pubmed]

submitter keyword: ubiquitination, Trypanosoma cruzi, proteasome

Susan Wyllie
contact affiliation	Mode of Action Group, BCDD, School of Life Sciences, University of Dundee
contact email	swyllie@dundee.ac.uk
lab head
Victoriano Corpas-Lopez
contact affiliation	University of Dundee
contact email	vcorpaslopez@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027524

PRIDE project URI

• PRIDE
  1. PXD027524
     1. Label: PRIDE project
     2. Name: Ubiquitination in treated T. cruzi parasites