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PXD027522

PXD027522 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAuxin profiling in Arabidopsis endoplasmic reticulum.
DescriptionEndoplasmic reticulum (ER) is an intracellular extensive network of membranes. Among major ER functions belong proteosynthesis, protein folding, their post-transcriptional modification and sorting within the cell and last but not least lipid anabolism. Moreover, several recent studies have indicated involvement of plant ER in regulation of intracellular auxin homeostasis by modulation of its metabolism. Therefore, in order to study auxin metabolome in ER, it is necessary to obtain highly enriched and, if possible, pure ER fraction. The separation of ER is troublesome due to similar biochemical properties with other cellular endomembranes. The majority of published protocols for ER isolation utilise density gradient ultracentrifugation despite the suboptimal resolving power of this approach. This work aims to optimize the isolation of ER from Arabidopsis thaliana for the subsequent determination of ER-specific auxin metabolite profiles by mass spectrometry. Following auxin metabolite determination showed a high increase of active auxin form within ER compared to whole plant. Moreover, our optimized isolation of ER may be further followed by multiple “omics” technologies including analysis of macromolecular as well as low molecular-weight compound from same sample.
HostingRepositoryPRIDE
AnnounceDate2021-11-09
AnnouncementXMLSubmission_2021-11-09_04:24:42.450.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD027522
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRené Lenobel
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListNo PTMs are included in the dataset
InstrumentmaXis
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-23 08:18:13ID requested
12021-11-09 04:24:42announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Endoplasmic reticulum, auxin, subcellular fractionation, density gradient centrifugation, mass spectrometry, Arabidopsis.
Contact List
Aleš Pěnčík
contact affiliationLaboratory of Growth Regulators, Institute of Experimental Botany of the Czech Academy of Sciences, and Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc CZ-78371, Czech Republic
contact emailales.pencik@upol.cz
lab head
René Lenobel
contact affiliationPalacky University in Olomouc, Department of protein biochemistry and proteomics
contact emailrene.lenobel@upol.cz
dataset submitter
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Dataset FTP location
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