PXD027522 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Auxin profiling in Arabidopsis endoplasmic reticulum. |
Description | Endoplasmic reticulum (ER) is an intracellular extensive network of membranes. Among major ER functions belong proteosynthesis, protein folding, their post-transcriptional modification and sorting within the cell and last but not least lipid anabolism. Moreover, several recent studies have indicated involvement of plant ER in regulation of intracellular auxin homeostasis by modulation of its metabolism. Therefore, in order to study auxin metabolome in ER, it is necessary to obtain highly enriched and, if possible, pure ER fraction. The separation of ER is troublesome due to similar biochemical properties with other cellular endomembranes. The majority of published protocols for ER isolation utilise density gradient ultracentrifugation despite the suboptimal resolving power of this approach. This work aims to optimize the isolation of ER from Arabidopsis thaliana for the subsequent determination of ER-specific auxin metabolite profiles by mass spectrometry. Following auxin metabolite determination showed a high increase of active auxin form within ER compared to whole plant. Moreover, our optimized isolation of ER may be further followed by multiple “omics” technologies including analysis of macromolecular as well as low molecular-weight compound from same sample. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-09 |
AnnouncementXML | Submission_2021-11-09_04:24:42.450.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD027522 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | René Lenobel |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | No PTMs are included in the dataset |
Instrument | maXis |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-23 08:18:13 | ID requested | |
⏵ 1 | 2021-11-09 04:24:42 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Endoplasmic reticulum, auxin, subcellular fractionation, density gradient centrifugation, mass spectrometry, Arabidopsis. |
Contact List
Aleš Pěnčík |
contact affiliation | Laboratory of Growth Regulators, Institute of Experimental Botany of the Czech Academy of Sciences, and Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc CZ-78371, Czech Republic |
contact email | ales.pencik@upol.cz |
lab head | |
René Lenobel |
contact affiliation | Palacky University in Olomouc, Department of protein biochemistry and proteomics |
contact email | rene.lenobel@upol.cz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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- PRIDE
- PXD027522
- Label: PRIDE project
- Name: Auxin profiling in Arabidopsis endoplasmic reticulum.