PXD027522

PXD027522 is an original dataset announced via ProteomeXchange.

Title	Auxin profiling in Arabidopsis endoplasmic reticulum.
Description	Endoplasmic reticulum (ER) is an intracellular extensive network of membranes. Among major ER functions belong proteosynthesis, protein folding, their post-transcriptional modification and sorting within the cell and last but not least lipid anabolism. Moreover, several recent studies have indicated involvement of plant ER in regulation of intracellular auxin homeostasis by modulation of its metabolism. Therefore, in order to study auxin metabolome in ER, it is necessary to obtain highly enriched and, if possible, pure ER fraction. The separation of ER is troublesome due to similar biochemical properties with other cellular endomembranes. The majority of published protocols for ER isolation utilise density gradient ultracentrifugation despite the suboptimal resolving power of this approach. This work aims to optimize the isolation of ER from Arabidopsis thaliana for the subsequent determination of ER-specific auxin metabolite profiles by mass spectrometry. Following auxin metabolite determination showed a high increase of active auxin form within ER compared to whole plant. Moreover, our optimized isolation of ER may be further followed by multiple “omics” technologies including analysis of macromolecular as well as low molecular-weight compound from same sample.
HostingRepository	PRIDE
AnnounceDate	2021-11-09
AnnouncementXML	Submission_2021-11-09_04:24:42.450.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027522
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	René Lenobel
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-23 08:18:13	ID requested
⏵ 1	2021-11-09 04:24:42	announced

Dataset with its publication pending

submitter keyword: Endoplasmic reticulum, auxin, subcellular fractionation, density gradient centrifugation, mass spectrometry, Arabidopsis.

Aleš Pěnčík
contact affiliation	Laboratory of Growth Regulators, Institute of Experimental Botany of the Czech Academy of Sciences, and Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc CZ-78371, Czech Republic
contact email	ales.pencik@upol.cz
lab head
René Lenobel
contact affiliation	Palacky University in Olomouc, Department of protein biochemistry and proteomics
contact email	rene.lenobel@upol.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD027522

PRIDE project URI

• PRIDE
  1. PXD027522
     1. Label: PRIDE project
     2. Name: Auxin profiling in Arabidopsis endoplasmic reticulum.