PXD027516

PXD027516 is an original dataset announced via ProteomeXchange.

Title	Syntaxin 5 determines Weibel-Palade body size and Von Willebrand factor secretion by controlling Golgi architecture
Description	Abstract Von Willebrand factor (VWF) is a multimeric hemostatic protein primarily synthesized in endothelial cells (ECs). VWF is stored in endothelial storage organelles, the Weibel-Palade bodies (WPBs), whose biogenesis strongly depends on VWF anterograde trafficking and Golgi architecture. Elongated WPB morphology is correlated to longer VWF strings with better adhesive properties. We previously identified the SNARE SEC22B, which is involved in anterograde ER-to-Golgi transport, as a novel regulator of WPB elongation. To elucidate novel determinants of WPB morphology we explored endothelial SEC22B interaction partners in a mass spectrometry-based approach, identifying the Golgi SNARE Syntaxin 5 (STX5). We established STX5 knockdown in ECs using shRNA-dependent silencing and analyzed WPB and Golgi morphology, using confocal and electron microscopy. STX5-depleted ECs exhibited extensive Golgi fragmentation and decreased WPB length, which was associated with reduced intracellular VWF levels, and impaired stimulated VWF secretion. However, the secretion incompetent organelles in shSTX5 cells maintained WPB markers such as Angiopoietin 2, P-selectin, Rab27A, and CD63. Taken together, our study has identified SNARE protein STX5 as a novel regulator of WPB biogenesis
HostingRepository	PRIDE
AnnounceDate	2022-01-27
AnnouncementXML	Submission_2022-01-27_07:18:25.213.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arie Hoogendijk
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-23 08:03:12	ID requested
⏵ 1	2022-01-27 07:18:25	announced

Kat M, Karampini E, Hoogendijk AJ, B, ü, rgisser PE, Mulder AA, Van Alphen FPJ, Olins J, Geerts D, Van den Biggelaar M, Margadant C, Voorberg J, Bierings R, Syntaxin 5 determines Weibel-Palade body size and von Willebrand factor secretion by controlling Golgi architecture. Haematologica, 107(8):1827-1839(2022) [pubmed]

submitter keyword: Human, Endothelial cells, Weibel-Palade bodies, Von Willebrand factor

Maartje van den Biggelaar
contact affiliation	Department of Molecular Hematology, Sanquin Research, Amsterdam, The Netherlands
contact email	m.vandenbiggelaar@sanquin.nl
lab head
Arie Hoogendijk
contact affiliation	Department of Molecular Hematology, Sanquin Research, Amsterdam
contact email	a.hoogendijk@sanquin.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027516
     1. Label: PRIDE project
     2. Name: Syntaxin 5 determines Weibel-Palade body size and Von Willebrand factor secretion by controlling Golgi architecture