PXD027507

PXD027507 is an original dataset announced via ProteomeXchange.

Title	A novel C1q domain-containing protein isolated from the bivalve mollusk Modiolus kurilensis recognizing glycans enriched with acidic galactans
Description	C1q domain-containing (C1qDC) proteins are a group of biopolymers involved in immune response as pattern recognition receptors (PRRs) in a lectin manner identifying and binding carbohydrate components of pathogen-associated molecular patterns (PAMPs). In the current study, we present a new protein MkC1qDC-1 purified from the hemolymph plasma of Modiolus kurilensis bivalve mollusk widespread in the Northwest Pacific. The isolation procedure included ammonium sulfate precipitation followed by affinity chromatography on pectin-Sepharose. The full-length MkC1qDC-1 sequence was assembled based on mass-spectrometry data supplemented with N-terminal Edman sequencing, and included 156 amino acid residues displaying high homology to C1qDC proteins of bivalves. 2-D electrophoresis of purified MkC1qDC-1 showed a single polypeptide with 19 kDa mass and pI 5.2 in accordance with predicted values. The protein was functionally active after 1 h incubation in temperatures up to 50°C and stable over a wide pH range. MkC1qDC-1 demonstrated antibacterial properties against gram-negative and gram-positive strains.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_05:34:20.099.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alex von kriegsheim
SpeciesList	scientific name: Modiolus kurilensis; NCBI TaxID: 1647520;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-22 06:08:42	ID requested
⏵ 1	2022-02-17 05:34:20	announced

Grinchenko AV, von Kriegsheim A, Shved NA, Egorova AE, Ilyaskina DV, Karp TD, Goncharov NV, Petrova IY, Kumeiko VV, Recognizing Glycans Enriched with Acidic Galactans and Mannans. Mar Drugs, 19(12):(2021) [pubmed]

submitter keyword: lc-ms/ms, de-novo sequencing

Alex von Kriegsheim
contact affiliation	university of edinburgh
contact email	avonkri@ed.ac.uk
lab head
Alex von kriegsheim
contact affiliation	University of Edinburgh
contact email	alex.vonkriegsheim@ucd.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027507
     1. Label: PRIDE project
     2. Name: A novel C1q domain-containing protein isolated from the bivalve mollusk Modiolus kurilensis recognizing glycans enriched with acidic galactans