PXD027475

PXD027475 is an original dataset announced via ProteomeXchange.

Title	Profiling of Subclass-Specific IgG Glycosylation in Gastric Cancer by nanoLC-ESI-MS/MS
Description	Background:Gastric cancer (GC) is one of the most aggressive gastrointestinal malignancy and leading cause of cancer related deaths worldwide,warranting the urgent discovery of biomarkers for early detection. IgG glycosylation is strongly related to disease progression and specific IgG glycans have been regarded as the next-generation biomarker, whereas it is rarly studied on the association between subclass-specific IgG glycosylation profile and GC progression. Methods:A total of 141 individuals were enrolled and randomly sorted into discovery set consisting of normal (N=30) and cancer patients (N=20), and validation set including controls (N=30), benign (N=25) and cancer (N=36).IgG glycopeptides were tryptic digested and enrichmed by microcrystalline cellulose based solid phase extraction.Subclass-specific IgG N-glycosylation was analyzed by nanoLC-MS/MS. Results:It was found that GC progression was associated with increased sialylation and decreased bisection of IgG2, as well as substantially decreased sialylation of IgG1 and increased galactosylation of IgG3/4.Further analysis of individual glycopeptides showed the changes of particular glycosylation of IgGwere reflected by the specific glycans taht could apparently distinguish different stages of gastric disease patients from normal. Conclusions:Different subclasses of IgG glycosylation were differentially expressed in GC, specific IgG glycopeptides could be promising biomarker for early detection of GC patients.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:28:48.242.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Si Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-21 07:53:03	ID requested
⏵ 1	2025-05-06 09:28:49	announced

10.1186/s12885-025-13616-z;

Xu T, Huang J, Lin J, Liu Y, Wang Y, Shen W, He J, Chen S, Zhu X, Que Y, Hu M, Chen Y, Cheng L, He H, Liu X, Liu S, Site-specific immunoglobulin G N-glycosylation is associated with gastric cancer progression. BMC Cancer, 25(1):217(2025) [pubmed]

submitter keyword: Human, Gastric cancer, Serum IgG,Subclass specific, LC-MS

Xin Liu
contact affiliation	Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, N0. 1037 Luoyudong Road, Hongshan District, Wuhan 430074
contact email	xliu@mail.hust.edu.cn
lab head
Si Liu
contact affiliation	Huazhong University of Science and Technology
contact email	siliu2019@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027475
     1. Label: PRIDE project
     2. Name: Profiling of Subclass-Specific IgG Glycosylation in Gastric Cancer by nanoLC-ESI-MS/MS