PXD027473

PXD027473 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Membrane proteome of HEK293T cells
Description	We developed a simple and effective method for “in-depth” membrane proteomics. We optimize the sample preparation for the maximum numbers of the identified membrane proteins using a human cell line, HEK293T, as a model. From this study, we demystified the merits of Urea wash methods for membrane enrichment. The membrane enrichment by Urea Wash was elucidated and compared to Alkaline Wash and without washing. The optimized protocol provides better comprehensiveness of the membrane proteome and enhances data quality.
HostingRepository	jPOST
AnnounceDate	2022-04-28
AnnouncementXML	Submission_2022-09-18_04:09:14.300.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shushi Nagamori
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; unknown modification
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-21 06:56:38	ID requested
1	2022-04-27 18:25:04	announced
⏵ 2	2022-09-18 04:09:15	announced	2022-09-18: Updated FTP location.

Publication List

Kongpracha P, Wiriyasermkul P, Isozumi N, Moriyama S, Kanai Y, Nagamori S, Simple But Efficacious Enrichment of Integral Membrane Proteins and Their Interactions for In-Depth Membrane Proteomics. Mol Cell Proteomics, 21(5):100206(2022) [pubmed]

Kongpracha P, Wiriyasermkul P, Isozumi N, Moriyama S, Kanai Y, Nagamori S, Simple But Efficacious Enrichment of Integral Membrane Proteins and Their Interactions for In-Depth Membrane Proteomics. Mol Cell Proteomics, 21(5):100206(2022) [pubmed]

Keyword List

submitter keyword: membrane protein, transporter, Urea Wash, trypsin digestion,

submitter keyword: membrane protein, transporter, Urea Wash, trypsin digestion,

Contact List

Shushi Nagamori
lab head
Shushi Nagamori
contact affiliation	The Jikei University School of Medicine
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001266/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001266/