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PXD027454

PXD027454 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA Novel Spectral Annotation Strategy Streamlines Reporting of mono-ADP-ribosylated Peptides Derived from Mouse Liver and Spleen in Response to IFN-gamma
DescriptionMass spectrometry-enabled ADP-ribosylation workflows are developing rapidly, providing researchers a variety of ADP-ribosylome enrichment strategies and mass spectrometric acquisition options. Despite the growth spurt in upstream technologies, systematic ADP-ribosyl (ADPr) peptide mass spectral annotation methods are lacking. HCD-dependent ADP-ribosylome studies are common but the resulting MS2 spectra are complex, owing to a mixture of b/y-ions and the m/p-ion peaks representing one or more dissociation events of the ADPr moiety (m-ion) and peptide (p-ion). In particular, p-ions can dominate HCD spectra but are not recognized by standard spectral annotation workflows. As a result, annotation strategies that are solely reliant upon the b/y-ions result in lower spectral scores that in turn reduce the number of reportable ADPr peptides. To improve the confidence of spectral assignments we implemented an ADPr peptide annotation and scoring strategy. All MS2 spectra are scored for the ADPr m-ions, but once spectra are assigned as an ADPr peptide they are further annotated and scored for the p-ions. We implemented this novel workflow to ADPr peptides enriched from the liver and spleen isolated from mice post 4-hour exposure to systemic IFN-gamma. HCD collision energy experiments were first performed on the Obitrap Fusion Lumos and the Q Exactive, with notable ADPr peptide dissociation properties verified with CID (Lumos). The m-ion and p-ion series score distributions revealed that ADPr peptide dissociation properties vary markedly between instruments and within instrument collision energy settings, with consequences on ADPr peptide reporting and amino acid localization.
HostingRepositoryPRIDE
AnnounceDate2021-11-02
AnnouncementXMLSubmission_2021-11-02_04:25:26.148.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD027454
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSasha Singh
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListadenosine diphosphoribosyl (ADP-ribosyl) modified residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-20 22:36:53ID requested
12021-11-02 04:25:26announced
Publication List
Kuraoka S, Higashi H, Yanagihara Y, Sonawane AR, Mukai S, Mlynarchik AK, Whelan MC, Hottiger MO, Nasir W, Delanghe B, Aikawa M, Singh SA, . Mol Cell Proteomics, 21(4):100153(2022) [pubmed]
Keyword List
submitter keyword: ADP-ribosylation, LC-MS/MS, IFN-gamma, Liver, Spleen, Software
Contact List
Masanori Aikawa
contact affiliationCenter for Interdisciplinary Cardiovascular Sciences, Brigham and Women’s Hospital, US
contact emailmaikawa@bwh.harvard.edu
lab head
Sasha Singh
contact affiliationBrigham and Women's Hospital, Harvard Medical School
contact emailsasingh@bwh.harvard.edu
dataset submitter
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Dataset FTP location
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